Detailed information of TA system TA057917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 806299..807235
• Replicon: plasmid C
• Accession: NZ_CP013110
• Organism: Sinorhizobium americanum strain CFNEI 73

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A1L3LWP1
• Locus tag: SAMCFNEI73_RS25315
• Protein ID: WP_064254220.1
• Coordinates: 806726..807235 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1L3LF60
• Locus tag: SAMCFNEI73_RS25310
• Protein ID: WP_037384278.1
• Coordinates: 806299..806670 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMCFNEI73_RS25290	802177..802560	+	384	WP_037384269.1	RidA family protein	-
SAMCFNEI73_RS25295	802577..804565	+	1989	WP_064254217.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
SAMCFNEI73_RS25300	804562..805431	+	870	WP_064254218.1	hydroxymethylglutaryl-CoA lyase	-
SAMCFNEI73_RS25305	805428..806213	+	786	WP_064254219.1	crotonase/enoyl-CoA hydratase family protein	-
SAMCFNEI73_RS25310	806299..806670	+	372	WP_037384278.1	DUF2384 domain-containing protein	Antitoxin
SAMCFNEI73_RS25315	806726..807235	+	510	WP_064254220.1	RES family NAD+ phosphorylase	Toxin
SAMCFNEI73_RS25320	807232..808596	-	1365	WP_064254221.1	FAD-binding oxidoreductase	-
SAMCFNEI73_RS25325	808736..809845	-	1110	WP_037384284.1	sulfate/molybdate ABC transporter ATP-binding protein	-
SAMCFNEI73_RS25330	809861..810745	-	885	WP_037384285.1	sulfate ABC transporter permease subunit CysW	-
SAMCFNEI73_RS25335	810735..811592	-	858	WP_064254222.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	katA / htpB	1..2177502	2177502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (8-162)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18752.25 Da
• Isoelectric Point: 6.0926

>T57917 WP_064254220.1 NZ_CP013110:806726-807235 [Sinorhizobium americanum]
MSPARAMTLWRAFVPRWAHMPLSGEGAARFGGRWNPVGTPTIYAARELSTAWAEYNQGFVQHPALIVQLELREAKLADLT
DGDVLAELGLEEAIHRCEWRDELDRGSVPQTHKAQAGLLKDGYDGVIYPSYMSPGGTCVALWRWNGRSGPRLDVIDPEGR
LPQSPASWL

Nucleotide

• Length: 510 bp

>T57917 NZ_CP013110:806726-807235 [Sinorhizobium americanum]
ATGTCGCCGGCGCGAGCGATGACGCTCTGGCGTGCCTTCGTGCCACGCTGGGCCCATATGCCGCTTTCCGGCGAGGGCGC
CGCCCGCTTCGGCGGCCGATGGAACCCCGTCGGAACGCCAACGATCTATGCGGCGCGGGAGCTTTCGACCGCTTGGGCCG
AATATAACCAGGGCTTCGTGCAGCACCCGGCGCTGATCGTGCAACTTGAGCTTCGCGAGGCGAAGCTGGCGGACCTGACG
GACGGCGATGTGCTTGCCGAACTCGGGCTCGAAGAGGCGATTCACCGCTGCGAGTGGCGCGACGAACTCGACAGGGGTTC
GGTGCCGCAGACGCACAAGGCCCAAGCCGGACTTTTGAAGGACGGCTACGACGGCGTGATCTACCCGTCCTACATGTCAC
CCGGCGGCACCTGTGTTGCGCTCTGGCGCTGGAACGGCCGGAGCGGGCCGCGGCTCGACGTCATCGATCCGGAAGGCCGG
CTGCCGCAATCGCCGGCCTCGTGGCTTTAG

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13143.29 Da
• Isoelectric Point: 9.7796

>AT57917 WP_037384278.1 NZ_CP013110:806299-806670 [Sinorhizobium americanum]
MMAVDFQIPAARFGDDHSPYLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAYDLVGEGKADKVLAYLEAIRAGVYA

Nucleotide

• Length: 372 bp

>AT57917 NZ_CP013110:806299-806670 [Sinorhizobium americanum]
ATGATGGCTGTCGATTTCCAGATTCCGGCGGCTCGCTTCGGTGACGATCATTCGCCGTACCTGTCGGCGCGCCTCGTTGC
CGACCGGCTCGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGCGTCGCCCGCAACACGCTGACGGCAAAGTCCGGCG
CGCGAAAGGTCGACAGCGCTTTGAGCAAGGTCGTTCGCATCCTCGCCATGGCGTCCGAAATGGCCGGCGACGAGGCCCGC
GCGGTCATCTGGTTCAAGCACCAGCCGATCCCCGGCTGGGCCGGCAAGACCGCCTATGATCTGGTCGGCGAAGGCAAGGC
CGACAAGGTCCTCGCTTATCTCGAGGCCATCCGCGCCGGCGTCTACGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1L3LWP1

Antitoxin

• AlphaFold DB: A0A1L3LF60