Detailed information of TA system TA057865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 41..653
• Replicon: chromosome
• Accession: NZ_CP013070
• Organism: Sphingobium indicum B90A

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: SIDU_RS00005
• Protein ID: WP_013041561.1
• Coordinates: 41..340 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: T0GLH9
• Locus tag: SIDU_RS00010
• Protein ID: WP_007682049.1
• Coordinates: 321..653 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SIDU_RS00005	41..340	+	300	WP_013041561.1	BrnT family toxin	Toxin
SIDU_RS00010	321..653	+	333	WP_007682049.1	BrnA antitoxin family protein	Antitoxin
SIDU_RS00015	1115..1810	-	696	Protein_2	IS5 family transposase	-
SIDU_RS19585	1972..2265	+	294	WP_145907151.1	hypothetical protein	-
SIDU_RS00020	2210..2878	-	669	WP_081440685.1	EthD domain-containing protein	-
SIDU_RS00025	2924..3685	-	762	WP_007682039.1	SDR family oxidoreductase	-
SIDU_RS00030	3732..4559	-	828	Protein_6	aromatic ring-hydroxylating dioxygenase subunit alpha	-
SIDU_RS00035	4621..4902	-	282	Protein_7	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4959..5723	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-90)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-99)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11439.76 Da
• Isoelectric Point: 6.2230

>T57865 WP_013041561.1 NZ_CP013070:41-340 [Sphingobium indicum B90A]
MRFSWHPAKAESNLRKHGISFETAASAFADPFALSVHDRIEGGEQRWQTLGLVEGHLLLLVAHTVAEDDDDGEYVEIIHI
ISARKADRKERRRYEEERR

Nucleotide

• Length: 300 bp

>T57865 NZ_CP013070:41-340 [Sphingobium indicum B90A]
ATGCGCTTTTCCTGGCACCCAGCCAAAGCCGAGAGCAATCTGAGAAAGCATGGCATCAGTTTCGAGACGGCCGCCAGCGC
CTTCGCAGACCCCTTTGCGCTTAGCGTGCATGATCGGATCGAAGGCGGCGAGCAACGATGGCAGACCTTGGGCCTGGTCG
AAGGACATTTACTCCTGCTGGTGGCCCACACCGTCGCTGAGGATGACGACGACGGCGAATATGTGGAGATCATCCATATC
ATTTCGGCCCGAAAAGCCGATCGAAAGGAAAGACGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12476.26 Da
• Isoelectric Point: 9.8217

>AT57865 WP_007682049.1 NZ_CP013070:321-653 [Sphingobium indicum B90A]
MKKKDADTVRFQLDPGNLPPLTEAQKAELDALQAMPDSGIDYSDAPTLTEDFWKTAERGRFYKPIKQQVTARLDADVLAW
LKSQGKGYQARMNAILRREMLAAAKERRHA

Nucleotide

• Length: 333 bp

>AT57865 NZ_CP013070:321-653 [Sphingobium indicum B90A]
ATGAAGAAGAAAGACGCTGATACCGTCCGCTTCCAGCTCGATCCGGGCAATCTGCCCCCCTTGACCGAGGCACAGAAGGC
CGAGCTGGATGCCCTGCAGGCGATGCCGGATAGCGGTATCGACTATAGTGATGCCCCGACGCTGACAGAGGATTTCTGGA
AGACCGCCGAGCGTGGACGGTTCTATAAGCCGATCAAGCAGCAGGTAACGGCGCGGCTCGACGCCGATGTGCTTGCTTGG
CTCAAATCCCAGGGCAAGGGCTATCAGGCTCGCATGAACGCAATCCTTCGGCGCGAGATGTTGGCGGCTGCGAAGGAGCG
CCGACACGCTTGA

Structures

Antitoxin

• AlphaFold DB: T0GLH9