57851

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 56732..57374 Replicon chromosome
Accession NZ_CP013055
Organism Bacillus thuringiensis strain YWC2-8

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag AQ980_RS00420 Protein ID WP_000635965.1
Coordinates 56732..57082 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AQ980_RS00425 Protein ID WP_000004570.1
Coordinates 57087..57374 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AQ980_RS00410 53667..54125 - 459 WP_000344239.1 SprT family protein -
AQ980_RS31895 54321..54437 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AQ980_RS00415 54496..56664 - 2169 WP_000426236.1 RNA-binding transcriptional accessory protein -
AQ980_RS00420 56732..57082 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AQ980_RS00425 57087..57374 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AQ980_RS00430 57683..58852 - 1170 WP_000390616.1 alanine racemase -
AQ980_RS00435 58970..59920 - 951 WP_002100659.1 outer membrane lipoprotein carrier protein LolA -
AQ980_RS00440 60077..60436 - 360 WP_000583417.1 holo-ACP synthase -
AQ980_RS00445 60530..61102 + 573 WP_000908522.1 rhomboid family intramembrane serine protease -
AQ980_RS00450 61095..62057 - 963 WP_000961167.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T57851 WP_000635965.1 NZ_CP013055:c57082-56732 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T57851 NZ_CP013055:c57082-56732 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT57851 WP_000004570.1 NZ_CP013055:c57374-57087 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT57851 NZ_CP013055:c57374-57087 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References