Detailed information of TA system TA057850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2177431..2178011
• Replicon: plasmid C
• Accession: NZ_CP013054
• Organism: Sinorhizobium americanum CCGM7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SAMCCGM7_RS31780
• Protein ID: WP_072597490.1
• Coordinates: 2177628..2178011 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R2C548
• Locus tag: SAMCCGM7_RS31775
• Protein ID: WP_037381623.1
• Coordinates: 2177431..2177631 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMCCGM7_RS31745	2172975..2173448	-	474	WP_027154429.1	isoprenylcysteine carboxylmethyltransferase family protein	-
SAMCCGM7_RS31750	2173470..2174363	-	894	WP_037381626.1	NAD(P)/FAD-dependent oxidoreductase	-
SAMCCGM7_RS31755	2174571..2175218	+	648	WP_037381625.1	TetR/AcrR family transcriptional regulator	-
SAMCCGM7_RS31760	2175253..2176116	+	864	WP_037381624.1	class I SAM-dependent methyltransferase	-
SAMCCGM7_RS33810	2176358..2176861	-	504	WP_037381662.1	SRPBCC family protein	-
SAMCCGM7_RS33815	2176845..2177200	-	356	Protein_2002	helix-turn-helix transcriptional regulator	-
SAMCCGM7_RS31775	2177431..2177631	+	201	WP_037381623.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SAMCCGM7_RS31780	2177628..2178011	+	384	WP_072597490.1	type II toxin-antitoxin system VapC family toxin	Toxin
SAMCCGM7_RS31785	2178444..2179142	-	699	WP_037381620.1	aspartate/glutamate racemase family protein	-
SAMCCGM7_RS31790	2179544..2180866	+	1323	WP_072597492.1	FAD-binding oxidoreductase	-
SAMCCGM7_RS31795	2180920..2182047	+	1128	WP_037381618.1	FAD-binding oxidoreductase	-
SAMCCGM7_RS31800	2182028..2182330	+	303	WP_037381616.1	(2Fe-2S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	katA / htpB	1..2249899	2249899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14168.24 Da
• Isoelectric Point: 6.8446

>T57850 WP_072597490.1 NZ_CP013054:2177628-2178011 [Sinorhizobium americanum CCGM7]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRSSVIAFLAAQREAFVATHDEVMIMIDRHAIFSM
GIGYTDAHLMASVLLDQRAALWTRDKRLRAAAERAGASLHTPANARN

Nucleotide

• Length: 384 bp

>T57850 NZ_CP013054:2177628-2178011 [Sinorhizobium americanum CCGM7]
GTGATACTTGCTGACACTTCCATATGGATTGATCATTTCAGGCATGCCGATTCAGAGCTGCGTAGGATCATAGAGGACGA
TCGTCTCCTCTGCCATCCGGCTGTGATTGGAGAACTCGCACTTGGCAGCCTTCGGGATCGTAGTAGCGTGATAGCTTTCC
TGGCGGCTCAGCGAGAAGCGTTCGTCGCAACGCACGACGAAGTCATGATCATGATTGATCGACACGCCATTTTCAGTATG
GGCATTGGCTACACAGATGCCCACTTGATGGCCTCAGTTCTCCTCGATCAGCGAGCGGCCTTGTGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCAGAAAGGGCGGGGGCTTCACTGCACACCCCTGCCAATGCGCGAAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7092.17 Da
• Isoelectric Point: 11.3760

>AT57850 WP_037381623.1 NZ_CP013054:2177431-2177631 [Sinorhizobium americanum CCGM7]
MRSTINLDDTLVERARSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT57850 NZ_CP013054:2177431-2177631 [Sinorhizobium americanum CCGM7]
ATGCGATCCACTATCAACCTCGACGACACCCTCGTGGAAAGGGCTAGGTCCCTAACCGGCACGAAAGAAACCGCCGCCCT
GGTCCGTCAAGCGTTGGAGACGCTTGTTCGTGTGGAGTCGGGAAAGCGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAGGCAGCCCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4R2C548