Detailed information of TA system TA057848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 491495..492165
• Replicon: plasmid C
• Accession: NZ_CP013054
• Organism: Sinorhizobium americanum CCGM7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1L3LCA8
• Locus tag: SAMCCGM7_RS23965
• Protein ID: WP_037382359.1
• Coordinates: 491746..492165 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SAMCCGM7_RS23960
• Protein ID: WP_037382358.1
• Coordinates: 491495..491749 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAMCCGM7_RS23940	486944..487522	-	579	Protein_462	TetR/AcrR family transcriptional regulator	-
SAMCCGM7_RS23945	487640..488863	+	1224	WP_037382355.1	MFS transporter	-
SAMCCGM7_RS23950	489027..489461	+	435	WP_037382356.1	OsmC family protein	-
SAMCCGM7_RS23955	489799..491280	+	1482	WP_037382357.1	trypsin-like peptidase domain-containing protein	-
SAMCCGM7_RS23960	491495..491749	+	255	WP_037382358.1	plasmid stabilization protein	Antitoxin
SAMCCGM7_RS23965	491746..492165	+	420	WP_037382359.1	type II toxin-antitoxin system VapC family toxin	Toxin
SAMCCGM7_RS23970	492406..492666	-	261	WP_037382360.1	hypothetical protein	-
SAMCCGM7_RS23975	492726..492908	-	183	WP_037382362.1	hypothetical protein	-
SAMCCGM7_RS23980	493016..493270	-	255	WP_037382363.1	type II toxin-antitoxin system ParD family antitoxin	-
SAMCCGM7_RS23985	493350..494141	-	792	WP_037382364.1	ATP-dependent Clp protease proteolytic subunit	-
SAMCCGM7_RS23990	494128..495435	-	1308	WP_037382365.1	hypothetical protein	-
SAMCCGM7_RS23995	495537..495800	-	264	WP_037382367.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	katA / htpB	1..2249899	2249899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14908.23 Da
• Isoelectric Point: 7.2020

>T57848 WP_037382359.1 NZ_CP013054:491746-492165 [Sinorhizobium americanum CCGM7]
MIVLDTNVVSEAMKPSPDPAVRSWLNEQVAESLYLSSVTMAELLFGVGALPDGRRKKALTETLHGVVELFGDRVLPFDIG
AARHYIELAVRARAAGKGFPTPAGYIAAIAASRGFLVATRDVSPFEAAGLPLVNPWAYR

Nucleotide

• Length: 420 bp

>T57848 NZ_CP013054:491746-492165 [Sinorhizobium americanum CCGM7]
ATGATCGTCTTGGATACGAATGTCGTCTCCGAAGCGATGAAACCATCTCCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
ACAGGTCGCTGAAAGCTTGTACCTGTCCAGTGTGACAATGGCCGAACTGCTATTTGGGGTCGGTGCGTTGCCCGATGGTC
GGCGCAAGAAGGCTCTGACCGAAACGCTTCACGGCGTGGTCGAACTGTTTGGTGATCGTGTATTGCCGTTCGACATCGGA
GCTGCTCGGCATTACATAGAACTGGCCGTGAGAGCGCGAGCGGCGGGAAAGGGATTTCCTACGCCCGCCGGATATATTGC
CGCCATTGCCGCCTCGCGTGGATTCCTCGTCGCGACGCGCGATGTCAGTCCCTTCGAAGCGGCGGGACTTCCCCTCGTGA
ATCCATGGGCCTATCGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9492.85 Da
• Isoelectric Point: 7.2069

>AT57848 WP_037382358.1 NZ_CP013054:491495-491749 [Sinorhizobium americanum CCGM7]
MAILTVRNVPEEVHRALRVRAATHGRSTEAEVRDILESVVKPAQRVRMGDALAELGRRLGLTNDDFVVFEQVRDKTPAEP
VRFE

Nucleotide

• Length: 255 bp

>AT57848 NZ_CP013054:491495-491749 [Sinorhizobium americanum CCGM7]
ATGGCCATCTTGACAGTTCGGAATGTACCCGAGGAAGTGCATCGCGCTTTGCGCGTGCGGGCGGCGACGCACGGTCGCAG
CACCGAGGCGGAAGTCCGCGATATTCTGGAAAGTGTGGTGAAGCCCGCGCAGCGCGTTCGCATGGGGGATGCCTTGGCCG
AACTCGGCCGGCGACTGGGCCTGACGAACGACGATTTCGTCGTGTTTGAGCAGGTACGTGACAAGACGCCGGCAGAGCCA
GTGAGGTTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A1L3LCA8