Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 6546..7186 Replicon plasmid A
Accession NZ_CP013052
Organism Sinorhizobium americanum CCGM7

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L3L8D9
Locus tag SAMCCGM7_RS17220 Protein ID WP_037381692.1
Coordinates 6546..6932 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L3L8D7
Locus tag SAMCCGM7_RS17225 Protein ID WP_037381694.1
Coordinates 6932..7186 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAMCCGM7_RS17195 2575..3789 + 1215 WP_037381687.1 replication initiation protein RepC -
SAMCCGM7_RS33505 3811..4406 - 596 Protein_3 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
SAMCCGM7_RS17205 4487..4759 - 273 WP_037381689.1 hypothetical protein -
SAMCCGM7_RS17210 4967..6142 - 1176 WP_052035435.1 site-specific integrase -
SAMCCGM7_RS17215 6213..6491 + 279 WP_037381691.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
SAMCCGM7_RS17220 6546..6932 - 387 WP_037381692.1 type II toxin-antitoxin system VapC family toxin Toxin
SAMCCGM7_RS17225 6932..7186 - 255 WP_037381694.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SAMCCGM7_RS17230 7967..9250 - 1284 WP_072597122.1 AAA family ATPase -
SAMCCGM7_RS17235 9231..11060 - 1830 WP_037381696.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..405481 405481


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-122)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 13955.82 Da        Isoelectric Point: 4.4898

>T57846 WP_037381692.1 NZ_CP013052:c6932-6546 [Sinorhizobium americanum CCGM7]
MVIDTSAIVAIAFNEPEAETYEQKVVDAPQRFLSAATLLELTIVIEARLGEAGTAELDLWLHKAGVEIVAVDAEQIAVAR
RAWRSYGKGRHPAGLNYGDCFSYALAKTRSEPLLYKGNDFSLTDIEAA

Download         Length: 387 bp

>T57846 NZ_CP013052:c6932-6546 [Sinorhizobium americanum CCGM7]
ATGGTCATCGATACGTCCGCGATCGTCGCGATCGCCTTCAATGAGCCGGAAGCGGAAACCTACGAGCAAAAGGTCGTCGA
CGCTCCGCAACGCTTTCTCTCGGCCGCCACGCTCCTGGAACTCACCATTGTCATCGAGGCCCGCCTCGGCGAGGCAGGGA
CCGCAGAGCTCGACCTGTGGCTCCATAAGGCCGGCGTCGAAATCGTAGCCGTCGATGCGGAACAGATCGCGGTGGCAAGG
CGAGCCTGGCGCAGCTATGGCAAGGGCCGACATCCGGCGGGGCTGAACTATGGGGACTGTTTCTCCTATGCGCTGGCCAA
GACACGCAGTGAACCGCTGCTCTATAAGGGGAATGATTTCTCGCTCACAGACATCGAGGCGGCATGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9517.89 Da        Isoelectric Point: 6.3783

>AT57846 WP_037381694.1 NZ_CP013052:c7186-6932 [Sinorhizobium americanum CCGM7]
MALSIKDLETERLAKALAEKTGETITMATRRALEERLRRLANVGNRDVLLEELAASRKRWARLPVLDNRSVDEILGYDEN
GLPN

Download         Length: 255 bp

>AT57846 NZ_CP013052:c7186-6932 [Sinorhizobium americanum CCGM7]
ATGGCGCTCAGCATTAAAGATCTAGAGACGGAACGCCTCGCAAAGGCACTGGCCGAGAAAACCGGCGAAACCATTACCAT
GGCGACCCGCCGGGCACTGGAGGAACGGCTGCGGCGCCTCGCAAACGTCGGAAATCGCGATGTCCTTCTCGAGGAGCTTG
CTGCCAGCCGTAAACGCTGGGCGAGGCTGCCTGTGCTCGACAACCGCTCTGTCGACGAGATTTTGGGGTATGACGAAAAC
GGGCTCCCCAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L3L8D9

Antitoxin

Source ID Structure
AlphaFold DB A0A1L3L8D7

References