Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1185402..1186023 Replicon chromosome
Accession NZ_CP013046
Organism Serratia marcescens strain B3R3

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag AR325_RS05640 Protein ID WP_004940313.1
Coordinates 1185402..1185605 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag AR325_RS05645 Protein ID WP_004940312.1
Coordinates 1185655..1186023 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AR325_RS05620 1180920..1182290 + 1371 WP_033633990.1 amidase -
AR325_RS05625 1182341..1184281 - 1941 WP_033633989.1 NAD(P)/FAD-dependent oxidoreductase -
AR325_RS26850 1184743..1185135 + 393 Protein_1079 cytochrome b/b6 domain-containing protein -
AR325_RS05640 1185402..1185605 - 204 WP_004940313.1 hemolysin expression modulator Hha Toxin
AR325_RS05645 1185655..1186023 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
AR325_RS05650 1186182..1186535 - 354 WP_004940311.1 hypothetical protein -
AR325_RS05655 1186940..1187653 + 714 WP_060560414.1 ABC transporter ATP-binding protein -
AR325_RS05660 1187650..1188507 + 858 WP_033633988.1 metal ABC transporter permease -
AR325_RS05665 1188532..1189410 + 879 WP_021504668.1 metal ABC transporter substrate-binding protein -
AR325_RS05670 1189519..1189659 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
AR325_RS05675 1189672..1189926 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T57820 WP_004940313.1 NZ_CP013046:c1185605-1185402 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T57820 NZ_CP013046:c1185605-1185402 [Serratia marcescens]
ATGACTAAAACCGACTATCTGATGCGTTTACGTAAATGCACCACGATTGATACTCTCGAACGTGTTATTGAGAAAAATAA
GTACGAGCTTTCCGATGATGAACTGGAGCTGTTTTACTCAGCGGCAGATCATCGTCTGGCAGAGCTCACCATGAACAAAC
TGTATGACAAAATCCCCACCTCCGTTTGGAAATATGTCAGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT57820 WP_004940312.1 NZ_CP013046:c1186023-1185655 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT57820 NZ_CP013046:c1186023-1185655 [Serratia marcescens]
ATGGATGAGTATTCGCCTAAGCGACACGACATTGCTCAGCTCAAATTCTTGTGCGAAAATTTGTATGATGAAGGTATCGC
TACCCTGGGAGACAGTCATCATGGGTGGGTCAACGATCCGACGTCCTCCGTCAACCTGCAGCTTAATGAGTTGATCGAAC
ATATCGCTTCGTTTGTGATGAGTTATAAAATTAAATACATGGACGAATCTGACCTGTCGGAGTTAGTCGAAGAATATCTC
GACGATACCTATACGCTGTTCAGCAGCTATGGTATCAATGATTCCGACCTGCGCCGCTGGCAAAAAACCAAGGCGAGATT
ATTCAGAATGTTCTCAGGAGAGGACATCTGTACGACAATGAAAACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References