Detailed information of TA system TA057818

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 84303..85069
• Replicon: chromosome
• Accession: NZ_CP013046
• Organism: Serratia marcescens strain B3R3

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0P0Q8U6
• Locus tag: AR325_RS00385
• Protein ID: WP_060558096.1
• Coordinates: 84701..85069 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AR325_RS00380
• Protein ID: WP_076606738.1
• Coordinates: 84303..84638 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AR325_RS00350	79651..79872	-	222	WP_060558082.1	AlpA family transcriptional regulator	-
AR325_RS00355	80335..81192	+	858	WP_060558089.1	hypothetical protein	-
AR325_RS00360	81289..82179	+	891	WP_076606737.1	50S ribosome-binding GTPase	-
AR325_RS00365	82427..82981	+	555	WP_060558091.1	hypothetical protein	-
AR325_RS00370	83009..83629	+	621	WP_060560398.1	hypothetical protein	-
AR325_RS00375	83811..84290	+	480	WP_060558094.1	DNA repair protein RadC	-
AR325_RS00380	84303..84638	+	336	WP_076606738.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AR325_RS00385	84701..85069	+	369	WP_060558096.1	TA system toxin CbtA family protein	Toxin
AR325_RS00390	85298..86152	+	855	WP_060560399.1	DUF4942 domain-containing protein	-
AR325_RS00395	86452..86643	+	192	WP_015376193.1	host cell division inhibitor Icd-like protein	-
AR325_RS00400	86640..86903	+	264	WP_033639063.1	hypothetical protein	-
AR325_RS00405	86900..87235	+	336	WP_033631431.1	DUF5375 family protein	-
AR325_RS00410	87246..89594	+	2349	WP_060558099.1	toprim domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13663.64 Da
• Isoelectric Point: 6.7085

>T57818 WP_060558096.1 NZ_CP013046:84701-85069 [Serratia marcescens]
MQTLPVTPKRAAYACPSPVVVWQSMLTYLLEQHYGLALNDTEFSDDTVIHEHIDAGISLADALNFTVEKFGLVRTDRRGF
SCQEQSPFITAIDILRARRATGLMTRMGYQTITSVIRGEKQA

Nucleotide

• Length: 369 bp

>T57818 NZ_CP013046:84701-85069 [Serratia marcescens]
ATGCAGACCTTACCTGTCACCCCGAAACGGGCGGCTTACGCTTGCCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTGGAGCAGCATTACGGCTTGGCGCTTAACGATACCGAGTTCAGTGATGACACCGTTATCCATGAACACATTG
ACGCCGGGATCTCTCTGGCTGATGCGTTGAATTTCACAGTGGAAAAGTTTGGCCTGGTTCGCACCGATCGCAGGGGGTTC
TCCTGTCAGGAACAATCCCCTTTTATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCTACCGGCCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGAAAAGCAGGCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12479.13 Da
• Isoelectric Point: 6.9656

>AT57818 WP_076606738.1 NZ_CP013046:84303-84638 [Serratia marcescens]
MSSKTTPAWGLKRNVTPQFGARLVQEGDRLHFLADRADISGKFSDEQVRDLNNAFPQFINHLELMLLSGELNPRYQHCVT
LYHKGLICEADSLGSHGYVYLAIYPTPQSTA

Nucleotide

• Length: 336 bp

>AT57818 NZ_CP013046:84303-84638 [Serratia marcescens]
ATGTCATCAAAAACTACCCCAGCCTGGGGACTTAAACGCAACGTCACTCCGCAGTTTGGTGCCCGCCTTGTACAAGAAGG
CGACCGGCTGCATTTTCTGGCCGACCGTGCCGATATCAGCGGGAAATTCAGTGACGAGCAGGTACGTGATTTAAATAACG
CCTTCCCGCAGTTTATCAACCACCTGGAACTGATGCTGCTCTCCGGTGAACTCAATCCACGTTATCAACACTGCGTCACG
CTCTACCACAAGGGACTAATCTGTGAAGCCGATAGTCTGGGTTCACACGGTTATGTGTATCTCGCCATTTATCCTACACC
CCAATCAACAGCATAA

Structures

Toxin

• AlphaFold DB: A0A0P0Q8U6