Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3807802..3808456 Replicon chromosome
Accession NZ_CP013031
Organism Escherichia coli strain H1827/12

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag AB847_RS18975 Protein ID WP_000244783.1
Coordinates 3807802..3808209 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AB847_RS18980 Protein ID WP_000354046.1
Coordinates 3808190..3808456 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AB847_RS18955 3803759..3805492 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
AB847_RS18960 3805498..3806208 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AB847_RS18965 3806233..3807129 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AB847_RS18970 3807241..3807762 + 522 WP_001055874.1 flavodoxin FldB -
AB847_RS18975 3807802..3808209 - 408 WP_000244783.1 protein YgfX Toxin
AB847_RS18980 3808190..3808456 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AB847_RS18985 3808699..3809679 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AB847_RS18990 3809875..3810534 - 660 WP_000250274.1 hemolysin III family protein -
AB847_RS18995 3810698..3811009 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
AB847_RS19000 3811054..3812487 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
AB847_RS19005 3812544..3813287 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T57813 WP_000244783.1 NZ_CP013031:c3808209-3807802 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T57813 NZ_CP013031:c3808209-3807802 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT57813 WP_000354046.1 NZ_CP013031:c3808456-3808190 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT57813 NZ_CP013031:c3808456-3808190 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References