Detailed information of TA system TA057789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 51815..52416
• Replicon: plasmid unnamed2
• Accession: NZ_CP013030
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: AKK22_RS27710
• Protein ID: WP_001216034.1
• Coordinates: 51815..52195 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: AKK22_RS27715
• Protein ID: WP_001190712.1
• Coordinates: 52195..52416 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS32945	46921..47151	-	231	Protein_51	ash family protein	-
AKK22_RS27685	47256..48740	-	1485	WP_000124150.1	terminase	-
AKK22_RS27690	48740..49933	-	1194	WP_000219616.1	hypothetical protein	-
AKK22_RS27695	50019..50471	-	453	WP_001326849.1	late promoter-activating protein	-
AKK22_RS27700	50560..51603	-	1044	WP_032326523.1	DUF968 domain-containing protein	-
AKK22_RS27705	51631..51810	-	180	WP_000113018.1	hypothetical protein	-
AKK22_RS27710	51815..52195	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AKK22_RS27715	52195..52416	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AKK22_RS27720	52489..52878	-	390	WP_032326524.1	DNA repair protein	-
AKK22_RS27725	53053..53625	+	573	WP_001133670.1	hypothetical protein	-
AKK22_RS27730	53632..53883	-	252	WP_001667237.1	DNA polymerase III subunit theta	-
AKK22_RS27735	54545..54907	-	363	WP_001261545.1	hypothetical protein	-
AKK22_RS27740	54904..55578	-	675	WP_000057458.1	hypothetical protein	-
AKK22_RS27745	55560..55934	-	375	WP_032326525.1	hypothetical protein	-
AKK22_RS27750	55941..56234	-	294	WP_000269004.1	hypothetical protein	-
AKK22_RS27755	56413..56646	-	234	WP_032326596.1	hypothetical protein	-
AKK22_RS27760	56729..57124	-	396	WP_000426669.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97704	97704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T57789 WP_001216034.1 NZ_CP013030:c52195-51815 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T57789 NZ_CP013030:c52195-51815 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAATTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT57789 WP_001190712.1 NZ_CP013030:c52416-52195 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT57789 NZ_CP013030:c52416-52195 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTTAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6