Detailed information of TA system TA057786

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4375143..4375589
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: AKK22_RS31395
• Protein ID: WP_072033237.1
• Coordinates: 4375143..4375265 (-)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: AKK22_RS23375
• Protein ID: WP_071887523.1
• Coordinates: 4375293..4375589 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS23345	4372166..4372735	-	570	WP_047619141.1	inovirus Gp2 family protein	-
AKK22_RS23360	4373099..4374261	+	1163	WP_085947598.1	IS3-like element IS3 family transposase	-
AKK22_RS31390	4374292..4375112	-	821	Protein_4327	transposase	-
AKK22_RS31395	4375143..4375265	-	123	WP_072033237.1	GhoT/OrtT family toxin	Toxin
AKK22_RS23375	4375293..4375589	-	297	WP_071887523.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
AKK22_RS23385	4375816..4376088	-	273	WP_032206017.1	N-acetyltransferase	-
AKK22_RS23390	4376100..4376317	-	218	Protein_4331	(4Fe-4S)-binding protein	-
AKK22_RS23395	4376498..4378129	+	1632	WP_001216477.1	sensor histidine kinase	-
AKK22_RS23400	4378126..4378845	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-36)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4687.74 Da
• Isoelectric Point: 10.8827

>T57786 WP_072033237.1 NZ_CP013029:c4375265-4375143 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSRLAP

Nucleotide

• Length: 123 bp

>T57786 NZ_CP013029:c4375265-4375143 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTAGACTGGCCCCCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11455.92 Da
• Isoelectric Point: 4.1705

>AT57786 WP_071887523.1 NZ_CP013029:c4375589-4375293 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGTPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT57786 NZ_CP013029:c4375589-4375293 [Escherichia coli]
ATGGAAGGTAAAAACAAATTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGACACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG