Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3911278..3911932 Replicon chromosome
Accession NZ_CP013029
Organism Escherichia coli strain 2012C-4227

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AKK22_RS20975 Protein ID WP_000244777.1
Coordinates 3911278..3911685 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AKK22_RS20980 Protein ID WP_000354046.1
Coordinates 3911666..3911932 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKK22_RS20955 3907236..3908969 - 1734 WP_032207675.1 single-stranded-DNA-specific exonuclease RecJ -
AKK22_RS20960 3908975..3909685 - 711 WP_000715227.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AKK22_RS20965 3909709..3910605 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AKK22_RS20970 3910717..3911238 + 522 WP_001055874.1 flavodoxin FldB -
AKK22_RS20975 3911278..3911685 - 408 WP_000244777.1 toxin CptA Toxin
AKK22_RS20980 3911666..3911932 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AKK22_RS20985 3912175..3913155 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AKK22_RS20990 3913351..3914010 - 660 WP_000250274.1 hemolysin III family protein -
AKK22_RS20995 3914174..3914484 - 311 Protein_3880 N(4)-acetylcytidine aminohydrolase -
AKK22_RS21000 3914529..3915962 + 1434 WP_032207745.1 6-phospho-beta-glucosidase BglA -
AKK22_RS21005 3916019..3916762 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T57783 WP_000244777.1 NZ_CP013029:c3911685-3911278 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T57783 NZ_CP013029:c3911685-3911278 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT57783 WP_000354046.1 NZ_CP013029:c3911932-3911666 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT57783 NZ_CP013029:c3911932-3911666 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References