Detailed information of TA system TA057780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3491955..3492580
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AKK22_RS18940
• Protein ID: WP_032206177.1
• Coordinates: 3491955..3492353 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AKK22_RS18945
• Protein ID: WP_000450524.1
• Coordinates: 3492353..3492580 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS18920	3487834..3488034	+	201	WP_000383836.1	YpfN family protein	-
AKK22_RS18925	3488144..3488841	-	698	Protein_3494	esterase	-
AKK22_RS18930	3488915..3490930	-	2016	WP_032206176.1	tRNA cytosine(34) acetyltransferase TmcA	-
AKK22_RS18935	3490945..3491808	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
AKK22_RS18940	3491955..3492353	-	399	WP_032206177.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AKK22_RS18945	3492353..3492580	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AKK22_RS18950	3492735..3493448	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AKK22_RS18960	3493661..3494695	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AKK22_RS18965	3494712..3495590	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AKK22_RS18970	3495736..3496308	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AKK22_RS18975	3496308..3496778	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14852.17 Da
• Isoelectric Point: 9.2216

>T57780 WP_032206177.1 NZ_CP013029:c3492353-3491955 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPGGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T57780 NZ_CP013029:c3492353-3491955 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGGTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT57780 WP_000450524.1 NZ_CP013029:c3492580-3492353 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT57780 NZ_CP013029:c3492580-3492353 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33