Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2231802..2232440 Replicon chromosome
Accession NZ_CP013029
Organism Escherichia coli strain 2012C-4227

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag AKK22_RS12350 Protein ID WP_000813794.1
Coordinates 2232264..2232440 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag AKK22_RS12345 Protein ID WP_001270286.1
Coordinates 2231802..2232218 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKK22_RS12325 2226954..2227895 - 942 WP_032206936.1 ABC transporter permease -
AKK22_RS12330 2227896..2228909 - 1014 WP_032206935.1 ABC transporter ATP-binding protein -
AKK22_RS12335 2228927..2230072 - 1146 WP_000047429.1 ABC transporter substrate-binding protein -
AKK22_RS12340 2230317..2231723 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
AKK22_RS12345 2231802..2232218 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
AKK22_RS12350 2232264..2232440 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
AKK22_RS12355 2232662..2232892 + 231 WP_071887485.1 YncJ family protein -
AKK22_RS12360 2232984..2234945 - 1962 Protein_2249 23S rRNA 5-hydroxycytidine C2501 synthase -
AKK22_RS12365 2235018..2235554 - 537 WP_032206933.1 DNA-binding transcriptional regulator SutR -
AKK22_RS12370 2235607..2236821 + 1215 WP_071887553.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2236861..2238009 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T57770 WP_000813794.1 NZ_CP013029:c2232440-2232264 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T57770 NZ_CP013029:c2232440-2232264 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGACGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT57770 WP_001270286.1 NZ_CP013029:c2232218-2231802 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT57770 NZ_CP013029:c2232218-2231802 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References