Detailed information of TA system TA057768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2057429..2057908
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A234Y3J1
• Locus tag: AKK22_RS11485
• Protein ID: WP_001444087.1
• Coordinates: 2057429..2057716 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A234Y267
• Locus tag: AKK22_RS11490
• Protein ID: WP_001090455.1
• Coordinates: 2057720..2057908 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS11450	2052653..2055115	-	2463	WP_060552853.1	exonuclease	-
AKK22_RS11455	2055208..2055396	-	189	WP_000199475.1	DUF1482 family protein	-
AKK22_RS11460	2055393..2055581	-	189	WP_000449175.1	cell division inhibitor	-
AKK22_RS11470	2056145..2056354	+	210	WP_001133037.1	hypothetical protein	-
AKK22_RS11475	2056355..2056993	-	639	WP_001473525.1	hypothetical protein	-
AKK22_RS11480	2057005..2057157	-	153	WP_000379546.1	DUF1391 family protein	-
AKK22_RS11485	2057429..2057716	-	288	WP_001444087.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AKK22_RS11490	2057720..2057908	-	189	WP_001090455.1	hypothetical protein	Antitoxin
AKK22_RS11495	2057938..2058336	-	399	WP_000209117.1	helix-turn-helix domain-containing protein	-
AKK22_RS29270	2058453..2058731	+	279	WP_000548554.1	helix-turn-helix domain-containing protein	-
AKK22_RS11500	2058715..2059236	+	522	WP_000705386.1	hypothetical protein	-
AKK22_RS11505	2059217..2060194	+	978	WP_000054521.1	hypothetical protein	-
AKK22_RS11510	2060201..2060941	+	741	WP_000790460.1	ATP-binding protein	-
AKK22_RS11515	2060971..2061741	+	771	WP_009453174.1	DUF1627 domain-containing protein	-
AKK22_RS11520	2061757..2062152	+	396	WP_001473635.1	DUF977 family protein	-
AKK22_RS11525	2062210..2062566	+	357	WP_009453172.1	hypothetical protein	-
AKK22_RS11530	2062617..2062829	+	213	WP_000935420.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2A / stx2B / nleC	2003799..2108375	104576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11014.71 Da
• Isoelectric Point: 6.2118

>T57768 WP_001444087.1 NZ_CP013029:c2057716-2057429 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVATLSIE
IVSVVHARRQFPKQN

Nucleotide

• Length: 288 bp

>T57768 NZ_CP013029:c2057716-2057429 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCAGAACGCTTATGGCAGCGATTAAGGGGGTGTGTGCTGCCGTTATCCGAACACCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCTCACCCTAACTATATAATTCTTTACCGTGTAGCCACTTTAAGCATTGAG
ATCGTGAGTGTAGTACACGCCAGACGCCAATTCCCCAAGCAGAACTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7249.25 Da
• Isoelectric Point: 8.6832

>AT57768 WP_001090455.1 NZ_CP013029:c2057908-2057720 [Escherichia coli]
MNTALSPMVSEFETIEQENSYNEWLRAKVAASLANPRPAIPHDEVERRMAERFAKMRKERSK

Nucleotide

• Length: 189 bp

>AT57768 NZ_CP013029:c2057908-2057720 [Escherichia coli]
ATGAATACGGCCCTTTCACCAATGGTTTCTGAGTTCGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAAATCCGCGCCCAGCAATTCCCCATGACGAGGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGTAAGTAG

Structures

Toxin

• AlphaFold DB: A0A234Y3J1

Antitoxin

• AlphaFold DB: A0A234Y267