Detailed information of TA system TA057765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 902293..903130
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: AKK22_RS05140
• Protein ID: WP_032208386.1
• Coordinates: 902293..902835 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: AKK22_RS05145
• Protein ID: WP_001297137.1
• Coordinates: 902819..903130 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS05120	897831..898742	-	912	WP_000705865.1	2-dehydropantoate 2-reductase	-
AKK22_RS05125	898910..899401	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
AKK22_RS05130	899529..900894	-	1366	Protein_875	MFS transporter	-
AKK22_RS05135	901302..902237	+	936	WP_032208388.1	sel1 repeat family protein	-
AKK22_RS05140	902293..902835	-	543	WP_032208386.1	GNAT family N-acetyltransferase	Toxin
AKK22_RS05145	902819..903130	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
AKK22_RS05150	903315..904205	-	891	WP_000971336.1	heme o synthase	-
AKK22_RS05155	904217..904546	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AKK22_RS05160	904546..905160	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AKK22_RS05165	905150..907141	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
AKK22_RS05170	907163..908110	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19943.14 Da
• Isoelectric Point: 8.0422

>T57765 WP_032208386.1 NZ_CP013029:c902835-902293 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPRQKQFDCGNPVIDKFVHESLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T57765 NZ_CP013029:c902835-902293 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTAGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACACGAATCACTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT57765 WP_001297137.1 NZ_CP013029:c903130-902819 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT57765 NZ_CP013029:c903130-902819 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCAAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3