Detailed information of TA system TA057763

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 323792..324495
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8T6BCS1
• Locus tag: AKK22_RS02160
• Protein ID: WP_001616568.1
• Coordinates: 324154..324495 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: AKK22_RS02155
• Protein ID: WP_032208435.1
• Coordinates: 323792..324133 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS02115	319251..319964	+	714	WP_032208428.1	transcriptional regulator	-
AKK22_RS02120	319961..320596	+	636	WP_001616559.1	hypothetical protein	-
AKK22_RS02125	320635..321090	+	456	WP_032208430.1	hypothetical protein	-
AKK22_RS02130	321087..321536	+	450	WP_032208432.1	hypothetical protein	-
AKK22_RS02135	321606..321836	+	231	WP_024188497.1	DUF905 domain-containing protein	-
AKK22_RS02140	321934..322755	+	822	WP_032208434.1	DUF945 domain-containing protein	-
AKK22_RS02145	322786..323226	+	441	WP_001616563.1	antirestriction protein	-
AKK22_RS02150	323239..323781	+	543	WP_001616564.1	DNA repair protein RadC	-
AKK22_RS02155	323792..324133	+	342	WP_032208435.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AKK22_RS02160	324154..324495	+	342	WP_001616568.1	TA system toxin CbtA family protein	Toxin
AKK22_RS02170	324743..325750	+	1008	WP_001616572.1	restriction endonuclease	-
AKK22_RS02180	326390..327576	+	1187	Protein_324	IS3-like element IS2 family transposase	-
AKK22_RS28330	327968..328090	+	123	WP_001300013.1	hypothetical protein	-
AKK22_RS02185	328098..329078	-	981	WP_032208436.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	296581..334116	37535

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12746.75 Da
• Isoelectric Point: 9.6548

>T57763 WP_001616568.1 NZ_CP013029:324154-324495 [Escherichia coli]
MKTLPATTPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T57763 NZ_CP013029:324154-324495 [Escherichia coli]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACGCCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTAAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.46 Da
• Isoelectric Point: 6.4660

>AT57763 WP_032208435.1 NZ_CP013029:323792-324133 [Escherichia coli]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLRLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYYNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT57763 NZ_CP013029:323792-324133 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAGGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACTACAATGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA