Detailed information of TA system TA057762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 49548..50107
• Replicon: plasmid unnamed1
• Accession: NZ_CP013028
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AKK22_RS00310
• Protein ID: WP_032206862.1
• Coordinates: 49832..50107 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: AKK22_RS00305
• Protein ID: WP_001178089.1
• Coordinates: 49548..49832 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS00270	45184..45396	+	213	WP_001302189.1	ANR family transcriptional regulator	-
AKK22_RS00275	45641..46102	+	462	WP_047619094.1	thermonuclease family protein	-
AKK22_RS00280	46148..46357	+	210	WP_047619093.1	gene expression modulator	-
AKK22_RS00285	46395..46985	+	591	WP_000766812.1	DUF2726 domain-containing protein	-
AKK22_RS00290	47225..47479	+	255	WP_000083830.1	replication regulatory protein RepA	-
AKK22_RS00295	47715..47789	+	75	WP_001370046.1	RepA leader peptide Tap	-
AKK22_RS00300	47782..48639	+	858	WP_032206860.1	incFII family plasmid replication initiator RepA	-
AKK22_RS00305	49548..49832	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
AKK22_RS00310	49832..50107	+	276	WP_032206862.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AKK22_RS00315	50202..50408	+	207	WP_001105064.1	hypothetical protein	-
AKK22_RS00325	50588..51765	-	1178	Protein_56	IS91 family transposase	-
AKK22_RS00330	51765..52130	-	366	WP_000091308.1	hypothetical protein	-
AKK22_RS00340	52167..53311	+	1145	WP_167588606.1	IS3 family transposase	-
AKK22_RS00345	53352..53723	+	372	Protein_59	chromosome partitioning protein ParB	-
AKK22_RS28155	53862..54035	-	174	Protein_60	DUF4113 domain-containing protein	-
AKK22_RS31575	53992..54156	+	165	Protein_61	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / espP / hlyD / hlyB / hlyA / hlyC	1..74656	74656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10680.34 Da
• Isoelectric Point: 9.5302

>T57762 WP_032206862.1 NZ_CP013028:49832-50107 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENS

Nucleotide

• Length: 276 bp

>T57762 NZ_CP013028:49832-50107 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT57762 WP_001178089.1 NZ_CP013028:49548-49832 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT57762 NZ_CP013028:49548-49832 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCTTGGACGATGTGACATCCGGACAGGTTATTGACCATCAAGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A731IHA8