Detailed information of TA system TA057761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 20374..20909
• Replicon: plasmid unnamed1
• Accession: NZ_CP013028
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: AKK22_RS00130
• Protein ID: WP_001270421.1
• Coordinates: 20374..20661 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: AKK22_RS00135
• Protein ID: WP_001132895.1
• Coordinates: 20658..20909 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS30555	16100..16920	-	821	Protein_16	transposase	-
AKK22_RS30565	17016..17236	+	221	Protein_17	serine/threonine protein kinase	-
AKK22_RS00115	17357..18097	+	741	WP_032208234.1	site-specific integrase	-
AKK22_RS00120	18340..19317	-	978	WP_032208235.1	RepB family plasmid replication initiator protein	-
AKK22_RS28115	19985..20372	-	388	Protein_20	DDE-type integrase/transposase/recombinase	-
AKK22_RS00130	20374..20661	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AKK22_RS00135	20658..20909	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AKK22_RS00140	21083..21268	-	186	WP_032208250.1	hypothetical protein	-
AKK22_RS00145	21433..22610	-	1178	Protein_24	IS91 family transposase	-
AKK22_RS00150	22610..22975	-	366	WP_000091308.1	hypothetical protein	-
AKK22_RS00155	23064..23254	-	191	Protein_26	IS3 family transposase	-
AKK22_RS00160	23489..25699	+	2211	WP_000592771.1	catalase/peroxidase KatP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	papC / espP / hlyD / hlyB / hlyA / hlyC	1..74656	74656
	-	inside	IScluster/Tn	-	-	14907..27028	12121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T57761 WP_001270421.1 NZ_CP013028:c20661-20374 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T57761 NZ_CP013028:c20661-20374 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCAGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT57761 WP_001132895.1 NZ_CP013028:c20909-20658 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT57761 NZ_CP013028:c20909-20658 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTGGACTTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6