Detailed information of TA system TA057760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 118757..119304
• Replicon: plasmid unnamed3
• Accession: NZ_CP013027
• Organism: Escherichia coli strain 2009C-3133

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: MJ49_RS27575
• Protein ID: WP_000969996.1
• Coordinates: 119023..119304 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: MJ49_RS27570
• Protein ID: WP_000079928.1
• Coordinates: 118757..119026 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ49_RS30320	113819..114022	+	204	WP_001327131.1	hypothetical protein	-
MJ49_RS27545	114765..115226	+	462	WP_000760078.1	thermonuclease family protein	-
-	115886..115947	-	62	NuclAT_1	-	-
-	115886..115947	-	62	NuclAT_1	-	-
-	115886..115947	-	62	NuclAT_1	-	-
-	115886..115947	-	62	NuclAT_1	-	-
MJ49_RS27945	115934..116140	+	207	WP_001553767.1	type I toxin-antitoxin system Hok family toxin	-
MJ49_RS27555	116424..116672	+	249	WP_000083835.1	replication regulatory protein RepA	-
MJ49_RS30340	116919..116993	+	75	WP_001365705.1	RepA leader peptide Tap	-
MJ49_RS27565	116986..117843	+	858	WP_000131010.1	incFII family plasmid replication initiator RepA	-
MJ49_RS27570	118757..119026	+	270	WP_000079928.1	hypothetical protein	Antitoxin
MJ49_RS27575	119023..119304	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJ49_RS27580	119389..119736	+	348	WP_001115718.1	hypothetical protein	-
MJ49_RS27585	119754..120344	-	591	WP_000194550.1	hypothetical protein	-
MJ49_RS27590	120344..120598	-	255	WP_000343103.1	hypothetical protein	-
MJ49_RS27595	121030..122238	-	1209	WP_000198533.1	IS91 family transposase	-
MJ49_RS27600	122219..122491	-	273	WP_000019163.1	hypothetical protein	-
MJ49_RS27605	122706..123521	+	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
MJ49_RS27610	123558..124136	+	579	Protein_134	DUF4238 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul2 / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B	espP / iucA / iucB / iucC / iucD / iutA / espP	1..174564	174564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T57760 WP_000969996.1 NZ_CP013027:119023-119304 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T57760 NZ_CP013027:119023-119304 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCACATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT57760 WP_000079928.1 NZ_CP013027:118757-119026 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT57760 NZ_CP013027:118757-119026 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGATCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGCGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCATCTGCTGCAGCATGGAGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A5W2J4D0