Detailed information of TA system TA057759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 105342..105967
• Replicon: plasmid unnamed3
• Accession: NZ_CP013027
• Organism: Escherichia coli strain 2009C-3133

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MJ49_RS27515
• Protein ID: WP_000911313.1
• Coordinates: 105342..105740 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: MJ49_RS27520
• Protein ID: WP_000450520.1
• Coordinates: 105740..105967 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ49_RS27500	101667..102164	+	498	WP_000605859.1	entry exclusion protein	-
MJ49_RS27505	102196..102927	+	732	WP_023565183.1	complement resistance protein TraT	-
MJ49_RS27510	103180..105333	+	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
MJ49_RS27515	105342..105740	-	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
MJ49_RS27520	105740..105967	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul2 / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B	espP / iucA / iucB / iucC / iucD / iutA / espP	1..174564	174564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T57759 WP_000911313.1 NZ_CP013027:c105740-105342 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T57759 NZ_CP013027:c105740-105342 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT57759 WP_000450520.1 NZ_CP013027:c105967-105740 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT57759 NZ_CP013027:c105967-105740 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2