Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1713057..1713554 Replicon chromosome
Accession NZ_CP013025
Organism Escherichia coli strain 2009C-3133

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MJ49_RS09390 Protein ID WP_001173343.1
Coordinates 1713057..1713230 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag MJ49_RS09395 Protein ID WP_001317561.1
Coordinates 1713258..1713554 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ49_RS09380 1709727..1711184 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
MJ49_RS09385 1711421..1712938 + 1518 WP_000003805.1 lysine--tRNA ligase -
MJ49_RS09390 1713057..1713230 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MJ49_RS09395 1713258..1713554 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MJ49_RS09405 1713781..1714053 - 273 WP_000405647.1 N-acetyltransferase -
MJ49_RS09410 1714065..1714295 - 231 WP_000371717.1 (4Fe-4S)-binding protein -
MJ49_RS09415 1714475..1716106 + 1632 WP_001216477.1 sensor histidine kinase -
MJ49_RS09420 1716103..1716822 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII 1646930..1713554 66624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T57725 WP_001173343.1 NZ_CP013025:c1713230-1713057 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T57725 NZ_CP013025:c1713230-1713057 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT57725 WP_001317561.1 NZ_CP013025:c1713554-1713258 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT57725 NZ_CP013025:c1713554-1713258 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References