Detailed information of TA system TA057709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 862806..863334
• Replicon: chromosome
• Accession: NZ_CP013013
• Organism: Vibrio cholerae strain Env-390

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: AP033_RS03970
• Protein ID: WP_000221352.1
• Coordinates: 863044..863334 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: AP033_RS03965
• Protein ID: WP_000213183.1
• Coordinates: 862806..863054 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AP033_RS03930	858542..859006	+	465	WP_000140244.1	hypothetical protein	-
AP033_RS03935	859106..859504	-	399	WP_001882293.1	hypothetical protein	-
AP033_RS03940	859660..860160	-	501	WP_000789723.1	hypothetical protein	-
AP033_RS03945	860298..860828	-	531	WP_000415563.1	hypothetical protein	-
AP033_RS03950	860979..861374	-	396	WP_000649527.1	DUF3465 domain-containing protein	-
AP033_RS03955	861582..862007	-	426	WP_000415748.1	hypothetical protein	-
AP033_RS03960	862275..862610	-	336	WP_000332173.1	hypothetical protein	-
AP033_RS03965	862806..863054	+	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AP033_RS03970	863044..863334	+	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AP033_RS03975	863470..863985	-	516	WP_001201532.1	lipocalin family protein	-
AP033_RS19125	864012..864104	-	93	WP_014164112.1	DUF3265 domain-containing protein	-
AP033_RS03980	864126..864704	-	579	WP_000110120.1	hypothetical protein	-
AP033_RS03985	864933..865175	+	243	WP_001107719.1	type II toxin-antitoxin system ParD family antitoxin	-
AP033_RS03990	865172..865471	+	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AP033_RS03995	865602..866117	-	516	WP_000100089.1	ClbS/DfsB family four-helix bundle protein	-
AP033_RS04000	866312..866494	-	183	WP_001889223.1	DUF645 family protein	-
AP033_RS04005	866789..867238	-	450	WP_000480555.1	hypothetical protein	-
AP033_RS04010	867373..867912	-	540	WP_000884561.1	hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	829699..942463	112764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T57709 WP_000221352.1 NZ_CP013013:863044-863334 [Vibrio cholerae]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T57709 NZ_CP013013:863044-863334 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT57709 WP_000213183.1 NZ_CP013013:862806-863054 [Vibrio cholerae]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT57709 NZ_CP013013:862806-863054 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8