Detailed information of TA system TA057699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 37782..38301
• Replicon: plasmid pWCF-TC1
• Accession: NZ_CP013010
• Organism: Enterococcus faecium strain UW7606x64/3 TC1

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: AQ614_RS13275
• Protein ID: WP_000588503.1
• Coordinates: 38044..38301 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: AQ614_RS13270
• Protein ID: WP_000388479.1
• Coordinates: 37782..38051 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQ614_RS13220	32979..33878	+	900	WP_002303312.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
AQ614_RS13225	33881..34366	+	486	WP_002303313.1	single-stranded DNA-binding protein	-
AQ614_RS13230	34507..34710	-	204	WP_002316074.1	putative holin-like toxin	-
AQ614_RS13235	34785..35045	+	261	WP_002295623.1	hypothetical protein	-
AQ614_RS13240	35482..35688	+	207	WP_002347218.1	hypothetical protein	-
AQ614_RS13245	35688..35939	+	252	WP_002296238.1	hypothetical protein	-
AQ614_RS13250	35954..36391	+	438	WP_002300851.1	hypothetical protein	-
AQ614_RS13255	36384..37091	+	708	WP_002303117.1	hypothetical protein	-
AQ614_RS13265	37472..37651	+	180	WP_001120991.1	hypothetical protein	-
AQ614_RS13270	37782..38051	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AQ614_RS13275	38044..38301	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
AQ614_RS14650	38760..38915	+	156	WP_002347142.1	hypothetical protein	-
AQ614_RS13280	38939..39763	+	825	WP_001809248.1	hypothetical protein	-
AQ614_RS13285	40001..41302	-	1302	WP_002302440.1	ISL3 family transposase	-
AQ614_RS13290	41429..42043	-	615	WP_001261742.1	recombinase family protein	-
AQ614_RS13295	42281..42703	+	423	WP_002303116.1	hypothetical protein	-
AQ614_RS13300	42713..42916	+	204	WP_002299573.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66496	66496

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T57699 WP_000588503.1 NZ_CP013010:38044-38301 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T57699 NZ_CP013010:38044-38301 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT57699 WP_000388479.1 NZ_CP013010:37782-38051 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT57699 NZ_CP013010:37782-38051 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7