Detailed information of TA system TA057696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/FR47-DUF1778
• Location: 46288..47084
• Replicon: plasmid pXcmN
• Accession: NZ_CP013007
• Organism: Xanthomonas citri pv. malvacearum strain XcmN1003

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: A0A6L9VEW6
• Locus tag: APY30_RS23640
• Protein ID: WP_033836093.1
• Coordinates: 46288..46797 (-)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A6P0G6J6
• Locus tag: APY30_RS23645
• Protein ID: WP_017154722.1
• Coordinates: 46794..47084 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APY30_RS23610	41327..41908	-	582	Protein_46	lytic transglycosylase	-
APY30_RS23625	42258..45437	+	3180	WP_089504609.1	TAL effector repeat-containing protein	-
APY30_RS23635	46025..46291	-	267	WP_033836092.1	hypothetical protein	-
APY30_RS23640	46288..46797	-	510	WP_033836093.1	GNAT family N-acetyltransferase	Toxin
APY30_RS23645	46794..47084	-	291	WP_017154722.1	DUF1778 domain-containing protein	Antitoxin
APY30_RS23650	47307..47531	+	225	WP_033836094.1	hypothetical protein	-
APY30_RS23655	47528..48091	+	564	WP_033836095.1	recombinase family protein	-
APY30_RS23660	48158..48808	+	651	WP_033836096.1	ParA family protein	-
APY30_RS23665	48808..49062	+	255	WP_012477434.1	hypothetical protein	-
APY30_RS24980	49285..49443	+	159	WP_190276501.1	hypothetical protein	-
APY30_RS23670	49440..50246	+	807	WP_033836097.1	serine protease	-
APY30_RS23675	50312..51232	+	921	WP_012477431.1	RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59644	59644
	-	flank	IS/Tn	-	-	47528..48091	563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 18189.82 Da
• Isoelectric Point: 8.0537

>T57696 WP_033836093.1 NZ_CP013007:c46797-46288 [Xanthomonas citri pv. malvacearum]
MKVSAPEPLAAHHDIEAFDSGVDSLDQWLKRRALKNQATGASRTFVACDDGRVVAYYALASSGVTVDAAPGRFRRNMPDP
IPVVVLGRLAVDQSQQGRGLGRALVKDAGQRIVQAADTIGIRGVLVHALSADAKAFYERIGFEPSPLDPMMLLVTLDDLK
ASLEPQARR

Nucleotide

• Length: 510 bp

>T57696 NZ_CP013007:c46797-46288 [Xanthomonas citri pv. malvacearum]
ATGAAGGTATCAGCGCCCGAACCGCTGGCCGCGCACCACGACATTGAAGCCTTCGACTCCGGGGTGGACAGTCTGGACCA
GTGGCTAAAGCGTCGTGCCCTGAAAAATCAGGCTACCGGCGCTTCGCGAACCTTCGTCGCCTGCGACGATGGCCGCGTGG
TGGCCTACTATGCGCTGGCATCGAGCGGGGTCACGGTGGACGCAGCGCCCGGACGCTTCCGACGCAACATGCCGGACCCG
ATTCCGGTGGTGGTCCTTGGCAGGCTGGCCGTAGATCAATCGCAGCAGGGCAGGGGCCTAGGCCGCGCTCTGGTGAAAGA
TGCCGGGCAGCGCATCGTCCAAGCCGCCGACACCATCGGGATTCGCGGGGTGCTCGTGCATGCGCTCTCGGCCGACGCCA
AGGCGTTCTATGAGCGGATCGGCTTTGAGCCGTCGCCCCTCGATCCGATGATGCTGCTGGTCACGCTGGATGACCTGAAA
GCCAGCCTCGAACCGCAAGCCCGCCGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10721.29 Da
• Isoelectric Point: 10.2633

>AT57696 WP_017154722.1 NZ_CP013007:c47084-46794 [Xanthomonas citri pv. malvacearum]
MPTMSTAPSKRETLNIRIKPEERSLIDRAAKARGKNRTDFVLEAARSAAEEALLDQTIISASPDAYAAFLARLDMPPQPN
ERLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT57696 NZ_CP013007:c47084-46794 [Xanthomonas citri pv. malvacearum]
ATGCCCACGATGAGCACCGCACCCAGCAAGCGTGAAACGCTGAACATCCGCATCAAGCCCGAGGAACGGAGCTTGATCGA
CCGCGCCGCCAAGGCACGGGGCAAGAACCGCACCGACTTCGTGCTGGAAGCCGCCCGGTCGGCCGCCGAGGAAGCGTTAC
TAGACCAGACGATCATTTCCGCCAGCCCGGACGCCTACGCGGCCTTTCTGGCACGACTGGACATGCCGCCGCAGCCGAAC
GAACGCCTGCGGAAAACCATGCAGACGCCCGCCCCGTGGGAGAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A6L9VEW6

Antitoxin

• AlphaFold DB: A0A6P0G6J6