Detailed information of TA system TA057695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 39645..40186
• Replicon: plasmid pXcmN
• Accession: NZ_CP013007
• Organism: Xanthomonas citri pv. malvacearum strain XcmN1003

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: APY30_RS23590
• Protein ID: WP_015462614.1
• Coordinates: 39645..39971 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: APY30_RS23595
• Protein ID: WP_003491150.1
• Coordinates: 39968..40186 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APY30_RS24975	34672..34839	+	168	WP_003490725.1	hypothetical protein	-
APY30_RS23555	35092..35445	-	354	WP_033836400.1	hypothetical protein	-
APY30_RS23560	35494..36201	-	708	WP_029820894.1	hypothetical protein	-
APY30_RS23565	36198..36644	-	447	WP_029221888.1	hypothetical protein	-
APY30_RS23570	36993..37388	+	396	WP_005931747.1	hypothetical protein	-
APY30_RS23575	37484..37753	+	270	WP_003490597.1	hypothetical protein	-
APY30_RS23580	37750..38043	+	294	WP_005931751.1	type II toxin-antitoxin system RelE/ParE family toxin	-
APY30_RS23590	39645..39971	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
APY30_RS23595	39968..40186	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
APY30_RS23600	40323..40697	-	375	WP_031425619.1	hypothetical protein	-
APY30_RS23605	40774..41193	-	420	WP_031425621.1	hypothetical protein	-
APY30_RS23610	41327..41908	-	582	Protein_46	lytic transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..59644	59644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T57695 WP_015462614.1 NZ_CP013007:c39971-39645 [Xanthomonas citri pv. malvacearum]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T57695 NZ_CP013007:c39971-39645 [Xanthomonas citri pv. malvacearum]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT57695 WP_003491150.1 NZ_CP013007:c40186-39968 [Xanthomonas citri pv. malvacearum]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT57695 NZ_CP013007:c40186-39968 [Xanthomonas citri pv. malvacearum]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7