Detailed information of TA system TA057680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4685342..4685958
• Replicon: chromosome
• Accession: NZ_CP012999
• Organism: Enterobacter sp. E20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: NI40_RS21925
• Protein ID: WP_080298642.1
• Coordinates: 4685342..4685713 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: NI40_RS21930
• Protein ID: WP_023309753.1
• Coordinates: 4685716..4685958 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NI40_RS21910	4682842..4683744	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
NI40_RS21915	4683741..4684376	+	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
NI40_RS21920	4684373..4685302	+	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
NI40_RS21925	4685342..4685713	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
NI40_RS21930	4685716..4685958	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
NI40_RS21935	4686158..4687078	+	921	WP_039264193.1	alpha/beta hydrolase	-
NI40_RS21940	4687087..4688028	-	942	WP_039264191.1	fatty acid biosynthesis protein FabY	-
NI40_RS21945	4688073..4688510	-	438	WP_039264190.1	D-tyrosyl-tRNA(Tyr) deacylase	-
NI40_RS21950	4688507..4689388	-	882	WP_033146965.1	virulence factor BrkB family protein	-
NI40_RS21955	4689382..4689981	-	600	WP_039264189.1	glucose-1-phosphatase	-
NI40_RS21960	4690070..4690540	-	471	WP_033146967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T57680 WP_080298642.1 NZ_CP012999:c4685713-4685342 [Enterobacter sp. E20]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T57680 NZ_CP012999:c4685713-4685342 [Enterobacter sp. E20]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGCGTAATTCTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT57680 WP_023309753.1 NZ_CP012999:c4685958-4685716 [Enterobacter sp. E20]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT57680 NZ_CP012999:c4685958-4685716 [Enterobacter sp. E20]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0