Detailed information of TA system TA057672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1643888..1644685
• Replicon: chromosome
• Accession: NZ_CP012999
• Organism: Enterobacter sp. E20

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: NI40_RS07640
• Protein ID: WP_176686754.1
• Coordinates: 1644185..1644685 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: NI40_RS07635
• Protein ID: WP_008499829.1
• Coordinates: 1643888..1644157 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NI40_RS07605	1639515..1639958	-	444	WP_039261676.1	helix-turn-helix transcriptional regulator	-
NI40_RS07610	1640111..1641352	+	1242	WP_039261681.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
NI40_RS07615	1641386..1641613	-	228	WP_008499832.1	YccJ family protein	-
NI40_RS07620	1641634..1642230	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
NI40_RS07625	1642621..1642791	+	171	WP_023311077.1	general stress protein	-
NI40_RS07630	1642909..1643826	+	918	WP_039261683.1	DMT family transporter	-
NI40_RS07635	1643888..1644157	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
NI40_RS07640	1644185..1644685	+	501	WP_176686754.1	GNAT family N-acetyltransferase	Toxin
NI40_RS07645	1644759..1646081	-	1323	WP_039261687.1	pyrimidine utilization transport protein G	-
NI40_RS07650	1646103..1646597	-	495	WP_039261688.1	pyrimidine utilization flavin reductase protein F	-
NI40_RS07655	1646607..1647197	-	591	WP_039261690.1	malonic semialdehyde reductase	-
NI40_RS07660	1647207..1648007	-	801	WP_032658084.1	pyrimidine utilization protein D	-
NI40_RS07665	1648015..1648401	-	387	WP_010429508.1	pyrimidine utilization protein C	-
NI40_RS07670	1648413..1649102	-	690	WP_033145167.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18969.67 Da
• Isoelectric Point: 7.9145

>T57672 WP_176686754.1 NZ_CP012999:1644185-1644685 [Enterobacter sp. E20]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMIPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T57672 NZ_CP012999:1644185-1644685 [Enterobacter sp. E20]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGTGTCCTGGGTTATT
ACACGTTATCGGGAAGCTGTTTTGAAAGAGCGATGATTCCTTCTAAGACTCAGCAGCGCCGAATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT57672 WP_008499829.1 NZ_CP012999:1643888-1644157 [Enterobacter sp. E20]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT57672 NZ_CP012999:1643888-1644157 [Enterobacter sp. E20]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43