Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1040750..1041370 Replicon chromosome
Accession NZ_CP012999
Organism Enterobacter sp. E20

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag NI40_RS04835 Protein ID WP_008499287.1
Coordinates 1040750..1040968 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag NI40_RS04840 Protein ID WP_008499288.1
Coordinates 1040996..1041370 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NI40_RS04810 1036764..1037024 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
NI40_RS22475 1037027..1037167 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
NI40_RS04815 1037164..1037874 - 711 WP_039263958.1 GNAT family N-acetyltransferase -
NI40_RS04820 1037976..1039436 + 1461 WP_039263959.1 PLP-dependent aminotransferase family protein -
NI40_RS04825 1039408..1039875 - 468 WP_023310598.1 hypothetical protein -
NI40_RS04830 1039993..1040544 - 552 WP_039263961.1 maltose O-acetyltransferase -
NI40_RS04835 1040750..1040968 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
NI40_RS04840 1040996..1041370 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
NI40_RS04845 1041882..1045028 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit -
NI40_RS04850 1045051..1046244 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T57670 WP_008499287.1 NZ_CP012999:c1040968-1040750 [Enterobacter sp. E20]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T57670 NZ_CP012999:c1040968-1040750 [Enterobacter sp. E20]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAGCTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT57670 WP_008499288.1 NZ_CP012999:c1041370-1040996 [Enterobacter sp. E20]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT57670 NZ_CP012999:c1041370-1040996 [Enterobacter sp. E20]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAATGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References