Detailed information of TA system TA057652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 93469..93991
• Replicon: plasmid pKpN06-SIL
• Accession: NZ_CP012994
• Organism: Klebsiella pneumoniae strain KpN06

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: AQD73_RS28090
• Protein ID: WP_004187019.1
• Coordinates: 93469..93753 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: AQD73_RS28095
• Protein ID: WP_004187025.1
• Coordinates: 93743..93991 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD73_RS28065	88688..89152	-	465	WP_004187010.1	hypothetical protein	-
AQD73_RS28075	90695..91219	+	525	Protein_89	hypothetical protein	-
AQD73_RS31625	91236..91719	+	484	Protein_90	hypothetical protein	-
AQD73_RS31630	91717..92016	+	300	Protein_91	conjugal transfer protein TraH	-
AQD73_RS28085	92098..93453	+	1356	WP_004187017.1	ISNCY family transposase	-
AQD73_RS28090	93469..93753	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AQD73_RS28095	93743..93991	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
AQD73_RS30265	94281..94705	-	425	Protein_95	IS1 family transposase	-
AQD73_RS30895	94893..95009	-	117	Protein_96	transposase domain-containing protein	-
AQD73_RS31560	94954..95217	-	264	WP_004187027.1	transposase	-
AQD73_RS31565	95232..95495	+	264	WP_004118208.1	hypothetical protein	-
AQD73_RS28110	95739..96020	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
AQD73_RS28115	96055..96624	+	570	WP_004152117.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..107110	107110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T57652 WP_004187019.1 NZ_CP012994:c93753-93469 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T57652 NZ_CP012994:c93753-93469 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT57652 WP_004187025.1 NZ_CP012994:c93991-93743 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT57652 NZ_CP012994:c93991-93743 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4