Detailed information of TA system TA057639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4580439..4581029
• Replicon: chromosome
• Accession: NZ_CP012992
• Organism: Klebsiella pneumoniae strain KpN06

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AQD73_RS22820
• Protein ID: WP_023341911.1
• Coordinates: 4580697..4581029 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: AQD73_RS22815
• Protein ID: WP_000288812.1
• Coordinates: 4580439..4580696 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD73_RS22795	4577039..4578388	-	1350	WP_077254247.1	DNA (cytosine-5-)-methyltransferase	-
AQD73_RS31390	4578396..4578812	-	417	WP_124047552.1	hypothetical protein	-
AQD73_RS31395	4579142..4579540	+	399	WP_115657462.1	ash family protein	-
AQD73_RS22805	4579440..4579901	+	462	WP_040210976.1	hypothetical protein	-
AQD73_RS22810	4579898..4580104	+	207	WP_022615589.1	helix-turn-helix domain-containing protein	-
AQD73_RS22815	4580439..4580696	+	258	WP_000288812.1	antitoxin	Antitoxin
AQD73_RS22820	4580697..4581029	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AQD73_RS22835	4581351..4582787	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
AQD73_RS22845	4583153..4584607	-	1455	WP_004148975.1	AMP nucleosidase	-
AQD73_RS22850	4584737..4584982	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T57639 WP_023341911.1 NZ_CP012992:4580697-4581029 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T57639 NZ_CP012992:4580697-4581029 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT57639 WP_000288812.1 NZ_CP012992:4580439-4580696 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT57639 NZ_CP012992:4580439-4580696 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0