Detailed information of TA system TA057628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 15913..16435
• Replicon: plasmid pKpN01-SIL
• Accession: NZ_CP012989
• Organism: Klebsiella pneumoniae strain KpN01

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: AQD68_RS27720
• Protein ID: WP_004187019.1
• Coordinates: 16151..16435 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: AQD68_RS27715
• Protein ID: WP_004187025.1
• Coordinates: 15913..16161 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD68_RS27690	13280..13849	-	570	WP_004152117.1	small heat shock protein sHSP20	-
AQD68_RS27695	13884..14165	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
AQD68_RS32220	14409..14672	-	264	WP_004118208.1	hypothetical protein	-
AQD68_RS32225	14687..14950	+	264	WP_004187027.1	transposase	-
AQD68_RS31020	14895..15011	+	117	Protein_17	transposase domain-containing protein	-
AQD68_RS30375	15199..15623	+	425	Protein_18	IS1 family transposase	-
AQD68_RS27715	15913..16161	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
AQD68_RS27720	16151..16435	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AQD68_RS27725	16451..17806	-	1356	WP_004187017.1	ISNCY family transposase	-
AQD68_RS32365	17888..18187	-	300	Protein_22	conjugal transfer protein TraH	-
AQD68_RS32370	18185..18668	-	484	Protein_23	hypothetical protein	-
AQD68_RS27735	18685..19209	-	525	Protein_24	hypothetical protein	-
AQD68_RS27745	20752..21216	+	465	WP_004187010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134064	134064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T57628 WP_004187019.1 NZ_CP012989:16151-16435 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T57628 NZ_CP012989:16151-16435 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT57628 WP_004187025.1 NZ_CP012989:15913-16161 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT57628 NZ_CP012989:15913-16161 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4