Detailed information of TA system TA057627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2282..3018
• Replicon: plasmid pKpN01-SIL
• Accession: NZ_CP012989
• Organism: Klebsiella pneumoniae strain KpN01

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: AQD68_RS27645
• Protein ID: WP_004187044.1
• Coordinates: 2282..2764 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: AQD68_RS27650
• Protein ID: WP_003026799.1
• Coordinates: 2752..3018 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD68_RS27640	725..2071	-	1347	WP_077251107.1	ISNCY family transposase	-
AQD68_RS27645	2282..2764	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
AQD68_RS27650	2752..3018	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
AQD68_RS27655	3169..3873	+	705	WP_060579418.1	IS6-like element IS26 family transposase	-
AQD68_RS27660	4032..6617	-	2586	WP_004187040.1	EAL domain-containing protein	-
AQD68_RS32215	6586..6831	-	246	WP_032238678.1	hypothetical protein	-
AQD68_RS27665	6889..7869	-	981	Protein_7	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134064	134064
	-	inside	IScluster/Tn	-	-	725..7869	7144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T57627 WP_004187044.1 NZ_CP012989:c2764-2282 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T57627 NZ_CP012989:c2764-2282 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT57627 WP_003026799.1 NZ_CP012989:c3018-2752 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT57627 NZ_CP012989:c3018-2752 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66