Detailed information of TA system TA057615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4587712..4588302
• Replicon: chromosome
• Accession: NZ_CP012987
• Organism: Klebsiella pneumoniae strain KpN01

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: AQD68_RS22860
• Protein ID: WP_023341911.1
• Coordinates: 4587970..4588302 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: AQD68_RS22855
• Protein ID: WP_000288812.1
• Coordinates: 4587712..4587969 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD68_RS22835	4584312..4585661	-	1350	WP_077254247.1	DNA (cytosine-5-)-methyltransferase	-
AQD68_RS31890	4585669..4586085	-	417	WP_124047552.1	hypothetical protein	-
AQD68_RS31895	4586415..4586813	+	399	WP_115657462.1	ash family protein	-
AQD68_RS22845	4586713..4587174	+	462	WP_040210976.1	hypothetical protein	-
AQD68_RS22850	4587171..4587377	+	207	WP_022615589.1	helix-turn-helix domain-containing protein	-
AQD68_RS22855	4587712..4587969	+	258	WP_000288812.1	antitoxin	Antitoxin
AQD68_RS22860	4587970..4588302	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AQD68_RS22875	4588624..4590060	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
AQD68_RS22885	4590426..4591880	-	1455	WP_004148975.1	AMP nucleosidase	-
AQD68_RS22890	4592010..4592255	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T57615 WP_023341911.1 NZ_CP012987:4587970-4588302 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T57615 NZ_CP012987:4587970-4588302 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT57615 WP_000288812.1 NZ_CP012987:4587712-4587969 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT57615 NZ_CP012987:4587712-4587969 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0