Detailed information of TA system TA057597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 272200..272777
• Replicon: chromosome
• Accession: NZ_CP012982
• Organism: Burkholderia cepacia ATCC 25416 strain UCB 717

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2G063
• Locus tag: APZ15_RS18645
• Protein ID: WP_021160897.1
• Coordinates: 272412..272777 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: APZ15_RS18640
• Protein ID: WP_021160898.1
• Coordinates: 272200..272418 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APZ15_RS18615	267462..267944	+	483	WP_027791292.1	GNAT family N-acetyltransferase	-
APZ15_RS18620	268032..269297	-	1266	WP_027791291.1	Zn-dependent hydrolase	-
APZ15_RS18625	269290..269814	-	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
APZ15_RS18630	269869..271035	-	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
APZ15_RS18635	271213..272109	+	897	WP_027791289.1	LysR family transcriptional regulator	-
APZ15_RS18640	272200..272418	+	219	WP_021160898.1	hypothetical protein	Antitoxin
APZ15_RS18645	272412..272777	+	366	WP_021160897.1	type II toxin-antitoxin system VapC family toxin	Toxin
APZ15_RS18650	273116..273679	+	564	WP_006488685.1	peroxiredoxin	-
APZ15_RS18655	273805..275403	+	1599	WP_027791288.1	alkyl hydroperoxide reductase subunit F	-
APZ15_RS41010	275508..276122	+	615	WP_155253146.1	hypothetical protein	-
APZ15_RS18665	276444..276998	-	555	WP_027791286.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13448.50 Da
• Isoelectric Point: 8.4389

>T57597 WP_021160897.1 NZ_CP012982:272412-272777 [Burkholderia cepacia ATCC 25416]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T57597 NZ_CP012982:272412-272777 [Burkholderia cepacia ATCC 25416]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT57597 WP_021160898.1 NZ_CP012982:272200-272418 [Burkholderia cepacia ATCC 25416]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT57597 NZ_CP012982:272200-272418 [Burkholderia cepacia ATCC 25416]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: U2G063

Antitoxin

• AlphaFold DB: U2HEK9