Detailed information of TA system TA057585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2008961..2009490
• Replicon: chromosome
• Accession: NZ_CP012979
• Organism: Staphylococcus aureus strain ST20130940

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAST40_RS09940
• Protein ID: WP_000621175.1
• Coordinates: 2008961..2009323 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAST40_RS09945
• Protein ID: WP_000948331.1
• Coordinates: 2009320..2009490 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST40_RS09915	2005940..2006710	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAST40_RS09920	2006685..2007164	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAST40_RS09925	2007166..2007492	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAST40_RS09930	2007611..2008612	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAST40_RS09940	2008961..2009323	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAST40_RS09945	2009320..2009490	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAST40_RS09950	2009575..2010723	-	1149	WP_000130547.1	alanine racemase	-
SAST40_RS09955	2010789..2011148	-	360	WP_000581202.1	holo-ACP synthase	-
SAST40_RS09960	2011152..2011643	-	492	WP_001205912.1	PH domain-containing protein	-
SAST40_RS09965	2011630..2013213	-	1584	WP_001294620.1	PH domain-containing protein	-
SAST40_RS09970	2013206..2013685	-	480	WP_001287079.1	hypothetical protein	-
SAST40_RS09975	2013894..2014454	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T57585 WP_000621175.1 NZ_CP012979:c2009323-2008961 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T57585 NZ_CP012979:c2009323-2008961 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT57585 WP_000948331.1 NZ_CP012979:c2009490-2009320 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT57585 NZ_CP012979:c2009490-2009320 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1