Detailed information of TA system TA057575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2008513..2009042
• Replicon: chromosome
• Accession: NZ_CP012978
• Organism: Staphylococcus aureus strain ST20130941

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAST41_RS09955
• Protein ID: WP_000621175.1
• Coordinates: 2008513..2008875 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAST41_RS09960
• Protein ID: WP_000948331.1
• Coordinates: 2008872..2009042 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST41_RS09930	2005492..2006262	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAST41_RS09935	2006237..2006716	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAST41_RS09940	2006718..2007044	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAST41_RS09945	2007163..2008164	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAST41_RS09955	2008513..2008875	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAST41_RS09960	2008872..2009042	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAST41_RS09965	2009127..2010275	-	1149	WP_000130547.1	alanine racemase	-
SAST41_RS09970	2010341..2010700	-	360	WP_000581202.1	holo-ACP synthase	-
SAST41_RS09975	2010704..2011195	-	492	WP_001205912.1	PH domain-containing protein	-
SAST41_RS09980	2011182..2012765	-	1584	WP_001294620.1	PH domain-containing protein	-
SAST41_RS09985	2012758..2013237	-	480	WP_001287079.1	hypothetical protein	-
SAST41_RS09990	2013446..2014006	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T57575 WP_000621175.1 NZ_CP012978:c2008875-2008513 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T57575 NZ_CP012978:c2008875-2008513 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT57575 WP_000948331.1 NZ_CP012978:c2009042-2008872 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT57575 NZ_CP012978:c2009042-2008872 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1