Detailed information of TA system TA057565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2060601..2061130
• Replicon: chromosome
• Accession: NZ_CP012976
• Organism: Staphylococcus aureus strain ST20130942

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAST42_RS10375
• Protein ID: WP_000621175.1
• Coordinates: 2060601..2060963 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAST42_RS10380
• Protein ID: WP_000948331.1
• Coordinates: 2060960..2061130 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST42_RS10350	2057579..2058349	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAST42_RS10355	2058324..2058803	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
SAST42_RS10360	2058805..2059131	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAST42_RS10365	2059250..2060251	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAST42_RS10375	2060601..2060963	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAST42_RS10380	2060960..2061130	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAST42_RS10385	2061215..2062363	-	1149	WP_062909809.1	alanine racemase	-
SAST42_RS10390	2062429..2062788	-	360	WP_000581200.1	holo-ACP synthase	-
SAST42_RS10395	2062792..2063283	-	492	WP_001205910.1	PH domain-containing protein	-
SAST42_RS10400	2063270..2064853	-	1584	WP_001294626.1	PH domain-containing protein	-
SAST42_RS10405	2064846..2065325	-	480	WP_001287088.1	hypothetical protein	-
SAST42_RS10410	2065534..2066094	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T57565 WP_000621175.1 NZ_CP012976:c2060963-2060601 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T57565 NZ_CP012976:c2060963-2060601 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT57565 WP_000948331.1 NZ_CP012976:c2061130-2060960 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT57565 NZ_CP012976:c2061130-2060960 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1