Detailed information of TA system TA057550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2060607..2061136
• Replicon: chromosome
• Accession: NZ_CP012974
• Organism: Staphylococcus aureus strain ST20130943

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAST43_RS10375
• Protein ID: WP_000621175.1
• Coordinates: 2060607..2060969 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAST43_RS10380
• Protein ID: WP_000948331.1
• Coordinates: 2060966..2061136 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST43_RS10350	2057585..2058355	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAST43_RS10355	2058330..2058809	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
SAST43_RS10360	2058811..2059137	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAST43_RS10365	2059256..2060257	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAST43_RS10375	2060607..2060969	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAST43_RS10380	2060966..2061136	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAST43_RS10385	2061221..2062369	-	1149	WP_062909809.1	alanine racemase	-
SAST43_RS10390	2062435..2062794	-	360	WP_000581200.1	holo-ACP synthase	-
SAST43_RS10395	2062798..2063289	-	492	WP_001205910.1	PH domain-containing protein	-
SAST43_RS10400	2063276..2064859	-	1584	WP_001294626.1	PH domain-containing protein	-
SAST43_RS10405	2064852..2065331	-	480	WP_001287088.1	hypothetical protein	-
SAST43_RS10410	2065540..2066100	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T57550 WP_000621175.1 NZ_CP012974:c2060969-2060607 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T57550 NZ_CP012974:c2060969-2060607 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT57550 WP_000948331.1 NZ_CP012974:c2061136-2060966 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT57550 NZ_CP012974:c2061136-2060966 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1