Detailed information of TA system TA057524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2057288..2057817
• Replicon: chromosome
• Accession: NZ_CP012970
• Organism: Staphylococcus aureus strain ST20130939

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAST39_RS10300
• Protein ID: WP_000621175.1
• Coordinates: 2057288..2057650 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAST39_RS10305
• Protein ID: WP_000948331.1
• Coordinates: 2057647..2057817 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST39_RS10275	2054267..2055037	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SAST39_RS10280	2055012..2055491	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAST39_RS10285	2055493..2055819	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAST39_RS10290	2055938..2056939	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAST39_RS10300	2057288..2057650	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAST39_RS10305	2057647..2057817	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAST39_RS10310	2057902..2059050	-	1149	WP_000130547.1	alanine racemase	-
SAST39_RS10315	2059116..2059475	-	360	WP_000581202.1	holo-ACP synthase	-
SAST39_RS10320	2059479..2059970	-	492	WP_001205912.1	PH domain-containing protein	-
SAST39_RS10325	2059957..2061540	-	1584	WP_001294620.1	PH domain-containing protein	-
SAST39_RS10330	2061533..2062012	-	480	WP_001287079.1	hypothetical protein	-
SAST39_RS10335	2062221..2062781	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T57524 WP_000621175.1 NZ_CP012970:c2057650-2057288 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T57524 NZ_CP012970:c2057650-2057288 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT57524 WP_000948331.1 NZ_CP012970:c2057817-2057647 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT57524 NZ_CP012970:c2057817-2057647 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1