Detailed information of TA system TA057509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 72920..73557
• Replicon: plasmid unnamed_4
• Accession: NZ_CP012965
• Organism: Cereibacter sphaeroides strain MBTLJ-8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: APX01_RS21520
• Protein ID: WP_011331362.1
• Coordinates: 72920..73327 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3IV80
• Locus tag: APX01_RS21525
• Protein ID: WP_011331361.1
• Coordinates: 73327..73557 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APX01_RS23150	69608..69775	-	168	Protein_67	Hsp20/alpha crystallin family protein	-
APX01_RS21515	69807..72431	-	2625	WP_017140473.1	cation-transporting P-type ATPase	-
APX01_RS23155	72440..72598	-	159	WP_017140474.1	SDR family oxidoreductase	-
APX01_RS21520	72920..73327	-	408	WP_011331362.1	tRNA(fMet)-specific endonuclease VapC	Toxin
APX01_RS21525	73327..73557	-	231	WP_011331361.1	antitoxin	Antitoxin
APX01_RS21530	73946..74779	-	834	WP_017140476.1	hypothetical protein	-
APX01_RS21535	74992..75417	-	426	WP_011331359.1	hypothetical protein	-
APX01_RS21540	75705..76130	+	426	WP_011331358.1	helix-turn-helix transcriptional regulator	-
APX01_RS21545	76694..77581	+	888	WP_011331357.1	viral aspartic protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / fcl	1..107170	107170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14801.00 Da
• Isoelectric Point: 6.8714

>T57509 WP_011331362.1 NZ_CP012965:c73327-72920 [Cereibacter sphaeroides]
MLRYMLDTNILIYTIRNRPGEVRAKFEAHHGTMCISAATAMELIYGAQKSQAVERNVAAVEGLLARLDILDLDLAAAQHA
GEIRAQLAQAGTPIGPFDVMIAGHARSKGLVLVSNNTREFERVDGLRLENWVQSS

Nucleotide

• Length: 408 bp

>T57509 NZ_CP012965:c73327-72920 [Cereibacter sphaeroides]
ATGCTGCGCTACATGCTCGACACGAACATCCTGATCTACACGATCAGGAACCGGCCCGGCGAGGTCAGGGCAAAGTTCGA
GGCCCATCACGGGACCATGTGCATCTCGGCCGCAACCGCGATGGAACTGATATACGGTGCGCAGAAATCGCAGGCCGTCG
AGCGCAACGTGGCGGCGGTCGAGGGACTCCTCGCGCGCCTCGACATTCTGGATCTCGATCTGGCGGCTGCCCAGCATGCC
GGGGAGATCCGGGCGCAGTTGGCGCAGGCAGGGACGCCCATCGGGCCGTTCGACGTGATGATCGCGGGACACGCGCGATC
TAAGGGCCTGGTCCTCGTGAGCAACAATACGCGGGAATTCGAACGCGTCGACGGCCTGAGACTGGAGAACTGGGTTCAGT
CGTCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8777.77 Da
• Isoelectric Point: 4.5600

>AT57509 WP_011331361.1 NZ_CP012965:c73557-73327 [Cereibacter sphaeroides]
VDRGSVFMNNRTQAIRLPKSVALPEGTRSVDIVRLGRAWLIVPSQDSWAQWFDEVSSSEDFMERRKQPDADERDDL

Nucleotide

• Length: 231 bp

>AT57509 NZ_CP012965:c73557-73327 [Cereibacter sphaeroides]
GTGGATCGGGGATCGGTCTTCATGAACAACCGGACACAGGCCATCCGCCTGCCAAAATCAGTTGCCTTGCCGGAAGGCAC
GCGGAGCGTCGACATCGTCCGGCTGGGCAGAGCCTGGCTCATCGTGCCGAGCCAGGACAGCTGGGCCCAATGGTTCGATG
AGGTCTCTTCGAGTGAAGACTTCATGGAGCGCCGGAAGCAACCGGACGCCGATGAGCGCGACGATCTCTGA

Structures

Antitoxin

• AlphaFold DB: Q3IV80