Detailed information of TA system TA057507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1410030..1410580
• Replicon: chromosome
• Accession: NZ_CP012960
• Organism: Cereibacter sphaeroides strain MBTLJ-8

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q3J2R5
• Locus tag: APX01_RS06785
• Protein ID: WP_002719915.1
• Coordinates: 1410030..1410278 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q3J2R4
• Locus tag: APX01_RS06790
• Protein ID: WP_011337726.1
• Coordinates: 1410275..1410580 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APX01_RS23325	1405443..1405736	+	294	WP_147175732.1	hypothetical protein	-
APX01_RS06755	1405733..1406521	+	789	WP_017140199.1	hypothetical protein	-
APX01_RS06760	1406536..1407190	+	655	Protein_1358	head decoration protein	-
APX01_RS06765	1407235..1407708	+	474	WP_017140200.1	hypothetical protein	-
APX01_RS06770	1407701..1408102	+	402	WP_011337723.1	hypothetical protein	-
APX01_RS06775	1408095..1408547	+	453	WP_002719913.1	hypothetical protein	-
APX01_RS06780	1408544..1408777	+	234	WP_011337724.1	tail protein X	-
APX01_RS06785	1410030..1410278	+	249	WP_002719915.1	BrnT family toxin	Toxin
APX01_RS06790	1410275..1410580	+	306	WP_011337726.1	BrnA antitoxin family protein	Antitoxin
APX01_RS06795	1411109..1411381	+	273	WP_011337727.1	hypothetical protein	-
APX01_RS22100	1411477..1412289	-	813	WP_081111710.1	hypothetical protein	-
APX01_RS23595	1412405..1412545	-	141	WP_002719918.1	hypothetical protein	-
APX01_RS06800	1412673..1412966	+	294	WP_011337728.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1400981..1412289	11308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (23-97)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8950.40 Da
• Isoelectric Point: 6.4624

>T57507 WP_002719915.1 NZ_CP012960:1410030-1410278 [Cereibacter sphaeroides]
MLVIVWDEPKRRSNLAKHGLDFADLDEEFFLSSVVVPASAGRHMAIGRLEDGTIAVVFAVLGAEGVSIISMRPASRKERS
LL

Nucleotide

• Length: 249 bp

>T57507 NZ_CP012960:1410030-1410278 [Cereibacter sphaeroides]
ATGTTGGTGATTGTATGGGATGAGCCAAAGCGGCGCAGCAACCTGGCGAAGCATGGGCTGGACTTCGCGGACCTAGACGA
GGAGTTCTTCCTGTCGTCGGTTGTCGTGCCAGCAAGCGCAGGCCGTCACATGGCGATCGGGCGCCTTGAGGATGGAACCA
TTGCCGTGGTCTTCGCGGTTCTGGGGGCCGAGGGCGTTTCGATCATCTCTATGCGCCCTGCGAGCCGAAAGGAGAGGAGC
CTTCTATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11061.61 Da
• Isoelectric Point: 10.5767

>AT57507 WP_011337726.1 NZ_CP012960:1410275-1410580 [Cereibacter sphaeroides]
MTKKRQLDTEETRIQRMIASDPDAPEATDAQLAQARPFTEAFPVLAEAMRRSAGGRPKLDAPKMSVSLRLDQDVVAHFKA
GGPGWQSRMNRALREAVGLSG

Nucleotide

• Length: 306 bp

>AT57507 NZ_CP012960:1410275-1410580 [Cereibacter sphaeroides]
ATGACGAAGAAGCGTCAACTCGACACGGAAGAGACCCGCATCCAGCGGATGATCGCGAGCGACCCGGATGCTCCCGAAGC
GACCGACGCGCAGCTTGCGCAGGCCCGACCCTTCACTGAGGCGTTTCCTGTGCTGGCCGAGGCCATGCGGCGCAGCGCAG
GCGGTCGCCCGAAGCTGGACGCGCCGAAAATGTCGGTTTCACTTCGCCTCGATCAGGACGTGGTGGCGCACTTCAAGGCA
GGCGGCCCCGGTTGGCAATCTCGGATGAACCGCGCCCTGCGGGAGGCGGTCGGCCTCTCCGGCTAG

Structures

Toxin

• AlphaFold DB: Q3J2R5

Antitoxin

• AlphaFold DB: Q3J2R4