Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 29464..30052 | Replicon | plasmid pD36-4 clone GC1 |
| Accession | NZ_CP012956 | ||
| Organism | Acinetobacter baumannii strain D36 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | A0A5N5XUQ1 |
| Locus tag | AN415_RS19760 | Protein ID | WP_047930174.1 |
| Coordinates | 29765..30052 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | A0A0J0ZQ33 |
| Locus tag | AN415_RS19755 | Protein ID | WP_047930176.1 |
| Coordinates | 29464..29778 (-) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AN415_RS19710 | 24954..25298 | + | 345 | WP_001986089.1 | helix-turn-helix domain-containing protein | - |
| AN415_RS19715 | 25471..25884 | + | 414 | Protein_29 | metallophosphoesterase | - |
| AN415_RS19720 | 26003..26686 | + | 684 | WP_000415733.1 | hypothetical protein | - |
| AN415_RS19725 | 26692..27225 | + | 534 | WP_047930179.1 | macro domain-containing protein | - |
| AN415_RS20365 | 27259..28349 | + | 1091 | WP_085940413.1 | IS4-like element ISAba1 family transposase | - |
| AN415_RS19740 | 28434..28820 | - | 387 | WP_000053135.1 | type II toxin-antitoxin system death-on-curing family toxin | - |
| AN415_RS19745 | 28817..28996 | - | 180 | WP_001231652.1 | hypothetical protein | - |
| AN415_RS19750 | 29046..29381 | - | 336 | WP_000689753.1 | hypothetical protein | - |
| AN415_RS19755 | 29464..29778 | - | 315 | WP_047930176.1 | BrnA antitoxin family protein | Antitoxin |
| AN415_RS19760 | 29765..30052 | - | 288 | WP_047930174.1 | BrnT family toxin | Toxin |
| AN415_RS19765 | 30314..31648 | - | 1335 | WP_001986494.1 | ISNCY-like element ISAba32 family transposase | - |
| AN415_RS19770 | 31780..32307 | - | 528 | WP_000537573.1 | plasmid pRiA4b ORF-3 family protein | - |
| AN415_RS19775 | 32522..32842 | + | 321 | Protein_40 | recombinase family protein | - |
| AN415_RS19780 | 33140..33955 | + | 816 | WP_001043260.1 | sulfonamide-resistant dihydropteroate synthase Sul2 | - |
| AN415_RS19785 | 34042..34284 | + | 243 | Protein_42 | phosphoglucosamine mutase | - |
| AN415_RS19790 | 34346..35050 | + | 705 | WP_001067786.1 | IS6 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | sul2 / aph(3')-Ia | - | 1..47457 | 47457 | |
| - | inside | IScluster/Tn | sul2 / aph(3')-Ia | - | 22404..44803 | 22399 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11276.65 Da Isoelectric Point: 5.6153
>T57489 WP_047930174.1 NZ_CP012956:c30052-29765 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG
MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISIQDRHTNGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG
Download Length: 288 bp
>T57489 NZ_CP012956:c30052-29765 [Acinetobacter baumannii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGATGATCCACTGAGGATCTCAATTCAGGATAGACATACCAATGGTGAAGAACGTTGGCAGACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTGTTTTTGATGATCCACTGAGGATCTCAATTCAGGATAGACATACCAATGGTGAAGAACGTTGGCAGACCATTGGAA
AAGTAAAAGGCGTACTAATGCTCTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACTAAGGCGGAGCGAGATAGATATGAGCATGGTTAG
Antitoxin
Download Length: 105 a.a. Molecular weight: 12285.00 Da Isoelectric Point: 9.4646
>AT57489 WP_047930176.1 NZ_CP012956:c29778-29464 [Acinetobacter baumannii]
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKKHQ
MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKKHQ
Download Length: 315 bp
>AT57489 NZ_CP012956:c29778-29464 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ACAATCAATTCTATCGACCAGTGAAGCAACAAACATCTATTCGTTTAGATGCTGACGTACTTGCATGGTTCAAAGCACAA
GGTAAAGGTTATCAGACACGCATGAATGAAATCTTGCGGGATGCCATGCTAAAAGAATTAAAAAAACATCAGTAA
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATGCCTGAAATTACCGACTGGAGCAATGCTGTACGCC
ACAATCAATTCTATCGACCAGTGAAGCAACAAACATCTATTCGTTTAGATGCTGACGTACTTGCATGGTTCAAAGCACAA
GGTAAAGGTTATCAGACACGCATGAATGAAATCTTGCGGGATGCCATGCTAAAAGAATTAAAAAAACATCAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5N5XUQ1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J0ZQ33 |