Detailed information of TA system TA057475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 1932904..1933514
• Replicon: plasmid unnamed
• Accession: NZ_CP012944
• Organism: Ralstonia solanacearum strain IBSBF1503

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F6G747
• Locus tag: RALBFv3_RS22985
• Protein ID: WP_014618391.1
• Coordinates: 1932904..1933212 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RALBFv3_RS22990
• Protein ID: WP_014618390.1
• Coordinates: 1933209..1933514 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RALBFv3_RS22975	1928015..1930732	-	2718	WP_014618393.1	type VI secretion system tip protein VgrG	-
RALBFv3_RS22980	1931629..1932828	+	1200	WP_003262834.1	MFS transporter	-
RALBFv3_RS22985	1932904..1933212	+	309	WP_014618391.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RALBFv3_RS22990	1933209..1933514	+	306	WP_014618390.1	putative addiction module antidote protein	Antitoxin
RALBFv3_RS22995	1933727..1935040	+	1314	WP_014618389.1	metabolite/H+ symporter	-
RALBFv3_RS23000	1935236..1936252	+	1017	WP_039548595.1	IS21 family transposase	-
RALBFv3_RS23005	1936249..1937037	+	789	WP_061581170.1	IS21-like element ISRso19 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / adeG / acrB / fliI / fliM / flgG / cyaB / cheY	1..2069788	2069788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11884.67 Da
• Isoelectric Point: 6.7111

>T57475 WP_014618391.1 NZ_CP012944:1932904-1933212 [Ralstonia solanacearum]
MLTIRTTELFDDWFCSLQDKAAQRRIQVRIDRLQMAHPGDMKAVRDDIRELRIDHGPGYRLYFVQHGVVLIVLLCGGAKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T57475 NZ_CP012944:1932904-1933212 [Ralstonia solanacearum]
ATGCTGACGATCCGCACGACCGAACTCTTTGACGACTGGTTTTGCAGCCTCCAAGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGCTCACCCGGGCGACATGAAAGCGGTACGTGACGACATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCTACCGCTTGTACTTCGTACAGCATGGCGTCGTCCTGATCGTACTGCTCTGCGGCGGCGCCAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11016.75 Da
• Isoelectric Point: 8.0667

>AT57475 WP_014618390.1 NZ_CP012944:1933209-1933514 [Ralstonia solanacearum]
MTMAQRIIKTRPWDSAEHLRTEADMAAYLNACLEEAGDDPALITHALGVVARARGMTRLARETGITREGLYKALSEDGNP
SFATMLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT57475 NZ_CP012944:1933209-1933514 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCATCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATGGCCGC
CTATCTCAACGCCTGCCTGGAAGAAGCCGGGGACGACCCGGCGCTCATTACGCACGCACTGGGCGTGGTCGCGCGTGCGC
GCGGCATGACCCGGCTCGCCCGCGAGACCGGGATCACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAACCCG
AGCTTTGCGACCATGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: F6G747