Detailed information of TA system TA057472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3356036..3356621
• Replicon: chromosome
• Accession: NZ_CP012943
• Organism: Ralstonia solanacearum strain IBSBF1503

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F6G268
• Locus tag: RALBFv3_RS15420
• Protein ID: WP_014617297.1
• Coordinates: 3356313..3356621 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F6G269
• Locus tag: RALBFv3_RS15415
• Protein ID: WP_014617298.1
• Coordinates: 3356036..3356320 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RALBFv3_RS15395	3351772..3352689	-	918	WP_014617302.1	MurR/RpiR family transcriptional regulator	-
RALBFv3_RS15400	3352857..3354146	+	1290	WP_014617301.1	FAD-dependent oxidoreductase	-
RALBFv3_RS15405	3354143..3355180	+	1038	WP_014617300.1	hypothetical protein	-
RALBFv3_RS15410	3355177..3356022	+	846	WP_014617299.1	TIM barrel protein	-
RALBFv3_RS15415	3356036..3356320	-	285	WP_014617298.1	putative addiction module antidote protein	Antitoxin
RALBFv3_RS15420	3356313..3356621	-	309	WP_014617297.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RALBFv3_RS15425	3356700..3357011	-	312	WP_014617296.1	Rieske 2Fe-2S domain-containing protein	-
RALBFv3_RS15430	3356977..3358134	-	1158	WP_052307006.1	fatty acid desaturase family protein	-
RALBFv3_RS15435	3358269..3359321	+	1053	WP_039549673.1	LacI family DNA-binding transcriptional regulator	-
RALBFv3_RS15440	3359531..3360448	+	918	WP_014617293.1	sugar ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11361.99 Da
• Isoelectric Point: 9.8179

>T57472 WP_014617297.1 NZ_CP012943:c3356621-3356313 [Ralstonia solanacearum]
MHTLIRSDTFDQWLEKLSDKRVKARITARLVSASFGNVGDCKPVGEGISEMRIDVGAGYRVYYTRQGTVVYILLTGGSKA
TQAQDIKQAIRMARELKESGHD

Nucleotide

• Length: 309 bp

>T57472 NZ_CP012943:c3356621-3356313 [Ralstonia solanacearum]
ATGCATACGCTGATCCGCTCCGACACGTTCGACCAGTGGCTTGAAAAGCTGAGCGACAAGCGCGTCAAGGCGCGGATCAC
CGCCCGGCTGGTCAGCGCATCATTCGGGAATGTTGGCGACTGCAAGCCCGTGGGGGAAGGCATCAGCGAAATGCGGATCG
ATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACCGTCGTCTACATCCTGCTCACGGGCGGCAGCAAAGCA
ACCCAAGCCCAGGATATCAAGCAAGCCATCCGGATGGCACGCGAGTTGAAGGAGTCAGGCCATGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9905.28 Da
• Isoelectric Point: 4.7361

>AT57472 WP_014617298.1 NZ_CP012943:c3356320-3356036 [Ralstonia solanacearum]
MTETYAPFDAAAYLDSEETIAEFLAAAMEDPNPDVFLAALGAVARARGIAKVAADAGLGRESLYKAISPGAHPRYETVQK
IVRALGVRLNVTPA

Nucleotide

• Length: 285 bp

>AT57472 NZ_CP012943:c3356320-3356036 [Ralstonia solanacearum]
ATGACTGAGACCTACGCACCGTTTGACGCGGCGGCCTATCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCCGCCGC
CATGGAAGACCCGAATCCGGACGTCTTCCTGGCTGCGCTCGGCGCCGTCGCGCGCGCCCGCGGCATTGCCAAGGTGGCCG
CGGACGCGGGCCTGGGGCGTGAAAGCCTGTACAAGGCCATCAGCCCGGGCGCACATCCACGCTACGAAACCGTCCAGAAA
ATCGTGCGCGCATTGGGCGTCAGGTTGAACGTGACGCCCGCTTGA

Structures

Toxin

• AlphaFold DB: F6G268

Antitoxin

• AlphaFold DB: F6G269