Detailed information of TA system TA057471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2970582..2971243
• Replicon: chromosome
• Accession: NZ_CP012943
• Organism: Ralstonia solanacearum strain IBSBF1503

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F6G2N5
• Locus tag: RALBFv3_RS13580
• Protein ID: WP_014617593.1
• Coordinates: 2970582..2970995 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F6G2N4
• Locus tag: RALBFv3_RS13585
• Protein ID: WP_014617592.1
• Coordinates: 2970992..2971243 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RALBFv3_RS13540	2965585..2966031	+	447	WP_014617601.1	hypothetical protein	-
RALBFv3_RS13545	2966139..2966912	+	774	WP_014617600.1	hypothetical protein	-
RALBFv3_RS13550	2966921..2967313	+	393	WP_014617599.1	hypothetical protein	-
RALBFv3_RS13555	2967310..2967531	+	222	WP_014617598.1	hypothetical protein	-
RALBFv3_RS13560	2967506..2968150	+	645	WP_014617597.1	hypothetical protein	-
RALBFv3_RS13565	2968193..2968483	-	291	WP_014617596.1	H-NS histone family protein	-
RALBFv3_RS13570	2968678..2970144	-	1467	WP_014617595.1	YOPP/AvrRxv family protein	-
RALBFv3_RS13575	2970344..2970577	+	234	WP_124994719.1	hypothetical protein	-
RALBFv3_RS13580	2970582..2970995	-	414	WP_014617593.1	type II toxin-antitoxin system VapC family toxin	Toxin
RALBFv3_RS13585	2970992..2971243	-	252	WP_014617592.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RALBFv3_RS13590	2971316..2975416	+	4101	WP_061581144.1	phage tail tape measure protein	-
RALBFv3_RS13595	2975422..2975817	+	396	WP_042549615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2927431..2991622	64191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15723.04 Da
• Isoelectric Point: 6.0853

>T57471 WP_014617593.1 NZ_CP012943:c2970995-2970582 [Ralstonia solanacearum]
MSYLVDTNVLSELRRKQPDAHVLAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLETELPNYFLGRLLDID
AHTADRWGRLMSSANRPLPAIDGLLAATALQHDLILVTRNTKDFVGLDVPLINPWET

Nucleotide

• Length: 414 bp

>T57471 NZ_CP012943:c2970995-2970582 [Ralstonia solanacearum]
TTGAGTTACCTGGTCGACACCAATGTCCTGTCCGAGCTACGCCGCAAGCAGCCCGATGCGCACGTGCTCGCGTGGATGCA
AGACCGGCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAAATCCGCAAAGGCATCGAGCGGCTGGACGACG
CAGCACGCCGCCAGCACCTGATCGACTGGCTCGAAACGGAACTGCCGAACTACTTCCTCGGCCGCTTGCTCGACATCGAT
GCCCATACCGCCGACCGCTGGGGCCGGCTGATGTCGAGCGCCAACCGGCCGCTGCCGGCCATCGACGGCCTGCTCGCCGC
GACCGCCCTGCAGCACGACCTCATACTGGTCACGCGTAACACGAAGGATTTCGTCGGACTCGACGTCCCGCTCATCAACC
CGTGGGAAACGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9256.49 Da
• Isoelectric Point: 5.0774

>AT57471 WP_014617592.1 NZ_CP012943:c2971243-2970992 [Ralstonia solanacearum]
MQSWQLQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVDFMRQSPLAGLDDVVFERERSLPRE
IEF

Nucleotide

• Length: 252 bp

>AT57471 NZ_CP012943:c2971243-2970992 [Ralstonia solanacearum]
ATGCAAAGCTGGCAGTTGCAAGCCGCCAAGGCGCGGTTTTCCGACGTCGTGAAGCGTGCAGCGGATGATGGTCCGCAGGA
AATCACGGTGCATGGCCGCCCGGTCGCAGTTGTGATCTCGCGCGCACTGTTCGATCGCCTGAGTGGCGGCGGTGAGTCGC
TGGTCGACTTCATGCGCCAGTCTCCGTTGGCTGGCCTGGACGACGTCGTCTTCGAGCGCGAACGCAGCCTGCCGCGCGAG
ATCGAGTTTTGA

Structures

Toxin

• AlphaFold DB: F6G2N5

Antitoxin

• AlphaFold DB: F6G2N4