Detailed information of TA system TA057470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1630874..1631507
• Replicon: chromosome
• Accession: NZ_CP012943
• Organism: Ralstonia solanacearum strain IBSBF1503

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F6FXL1
• Locus tag: RALBFv3_RS07410
• Protein ID: WP_014615899.1
• Coordinates: 1630874..1631287 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F6FXL0
• Locus tag: RALBFv3_RS07415
• Protein ID: WP_003263271.1
• Coordinates: 1631274..1631507 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RALBFv3_RS07390	1626209..1627945	+	1737	WP_003266149.1	ABC transporter substrate-binding protein	-
RALBFv3_RS07395	1628117..1629613	+	1497	WP_014615902.1	glycerol kinase GlpK	-
RALBFv3_RS07400	1629688..1630062	-	375	WP_039546861.1	hypothetical protein	-
RALBFv3_RS07405	1630273..1630899	+	627	WP_014615900.1	DUF1415 family protein	-
RALBFv3_RS07410	1630874..1631287	-	414	WP_014615899.1	PIN domain-containing protein	Toxin
RALBFv3_RS07415	1631274..1631507	-	234	WP_003263271.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RALBFv3_RS07420	1631565..1633172	-	1608	WP_039567295.1	glucan biosynthesis protein	-
RALBFv3_RS07425	1633368..1634636	+	1269	WP_124994693.1	AGE family epimerase/isomerase	-
RALBFv3_RS07430	1634644..1635933	+	1290	WP_003263268.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-40)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14919.23 Da
• Isoelectric Point: 5.9701

>T57470 WP_014615899.1 NZ_CP012943:c1631287-1630874 [Ralstonia solanacearum]
MPAAEAFFDSNIVLYLLSADAAKADAAEALLMAGGVVSVQVLNETTHVMRRKLAMPWHAIEAVQEAVRAQCRVEPLTVET
HDLGRHLAQRYGLSVYDALIVAAASLAGCRVLYSEDMQHGLVIEQRLRVVNPFFASA

Nucleotide

• Length: 414 bp

>T57470 NZ_CP012943:c1631287-1630874 [Ralstonia solanacearum]
ATGCCCGCGGCTGAGGCGTTTTTCGACAGCAACATCGTGCTGTACCTGCTGTCGGCCGATGCCGCCAAGGCCGATGCGGC
GGAAGCGCTACTGATGGCCGGCGGCGTCGTCAGCGTGCAGGTGCTCAACGAAACGACGCACGTGATGCGCCGCAAGCTCG
CCATGCCGTGGCACGCGATCGAAGCGGTGCAGGAAGCCGTACGCGCGCAATGCCGGGTCGAGCCGCTGACGGTCGAAACC
CATGATCTCGGGCGGCATCTTGCGCAGCGCTATGGATTGTCGGTCTATGACGCGCTGATCGTCGCCGCAGCGTCGCTGGC
CGGTTGCCGCGTCCTGTATTCCGAGGACATGCAGCACGGCCTGGTGATCGAGCAGCGTCTGCGCGTCGTCAATCCTTTTT
TCGCGTCGGCGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8664.82 Da
• Isoelectric Point: 8.7288

>AT57470 WP_003263271.1 NZ_CP012943:c1631507-1631274 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALGLHEGDDVEVVVAGERSFGVRRKRSARELFERLHQYRGKLPADFIFERDDANARG

Nucleotide

• Length: 234 bp

>AT57470 NZ_CP012943:c1631507-1631274 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCCATCCGGCTGCCCGCAGCCGTTGTGGAGGCGCTTGGACTGCACGAAGG
CGACGACGTCGAGGTGGTGGTGGCGGGCGAACGCTCGTTTGGTGTGCGCCGCAAGCGTTCCGCGCGTGAGTTGTTTGAGC
GGCTCCACCAATACCGCGGCAAGCTGCCCGCCGACTTCATCTTCGAGCGCGACGACGCCAATGCCCGCGGCTGA

Structures

Toxin

• AlphaFold DB: F6FXL1

Antitoxin

• AlphaFold DB: F6FXL0