Detailed information of TA system TA057467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2761572..2762157
• Replicon: chromosome
• Accession: NZ_CP012939
• Organism: Ralstonia solanacearum strain UW163

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F6G268
• Locus tag: UW163_RS12880
• Protein ID: WP_014617297.1
• Coordinates: 2761849..2762157 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F6G269
• Locus tag: UW163_RS12875
• Protein ID: WP_014617298.1
• Coordinates: 2761572..2761856 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UW163_RS12855	2757308..2758225	-	918	WP_014617302.1	MurR/RpiR family transcriptional regulator	-
UW163_RS12860	2758393..2759682	+	1290	WP_014617301.1	FAD-dependent oxidoreductase	-
UW163_RS12865	2759679..2760716	+	1038	WP_014617300.1	hypothetical protein	-
UW163_RS12870	2760713..2761558	+	846	WP_014617299.1	TIM barrel protein	-
UW163_RS12875	2761572..2761856	-	285	WP_014617298.1	putative addiction module antidote protein	Antitoxin
UW163_RS12880	2761849..2762157	-	309	WP_014617297.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
UW163_RS12885	2762236..2762547	-	312	WP_014617296.1	Rieske 2Fe-2S domain-containing protein	-
UW163_RS12890	2762513..2763670	-	1158	WP_052491361.1	fatty acid desaturase family protein	-
UW163_RS12895	2763805..2764857	+	1053	WP_039549673.1	LacI family DNA-binding transcriptional regulator	-
UW163_RS12900	2765067..2765984	+	918	WP_014617293.1	sugar ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11361.99 Da
• Isoelectric Point: 9.8179

>T57467 WP_014617297.1 NZ_CP012939:c2762157-2761849 [Ralstonia solanacearum]
MHTLIRSDTFDQWLEKLSDKRVKARITARLVSASFGNVGDCKPVGEGISEMRIDVGAGYRVYYTRQGTVVYILLTGGSKA
TQAQDIKQAIRMARELKESGHD

Nucleotide

• Length: 309 bp

>T57467 NZ_CP012939:c2762157-2761849 [Ralstonia solanacearum]
ATGCATACGCTGATCCGCTCCGACACGTTCGACCAGTGGCTTGAAAAGCTGAGCGACAAGCGCGTCAAGGCGCGGATCAC
CGCCCGGCTGGTCAGCGCATCATTCGGGAATGTTGGCGACTGCAAGCCCGTGGGGGAAGGCATCAGCGAAATGCGGATCG
ATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACCGTCGTCTACATCCTGCTCACGGGCGGCAGCAAAGCA
ACCCAAGCCCAGGATATCAAGCAAGCCATCCGGATGGCACGCGAGTTGAAGGAGTCAGGCCATGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9905.28 Da
• Isoelectric Point: 4.7361

>AT57467 WP_014617298.1 NZ_CP012939:c2761856-2761572 [Ralstonia solanacearum]
MTETYAPFDAAAYLDSEETIAEFLAAAMEDPNPDVFLAALGAVARARGIAKVAADAGLGRESLYKAISPGAHPRYETVQK
IVRALGVRLNVTPA

Nucleotide

• Length: 285 bp

>AT57467 NZ_CP012939:c2761856-2761572 [Ralstonia solanacearum]
ATGACTGAGACCTACGCACCGTTTGACGCGGCGGCCTATCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCCGCCGC
CATGGAAGACCCGAATCCGGACGTCTTCCTGGCTGCGCTCGGCGCCGTCGCGCGCGCCCGCGGCATTGCCAAGGTGGCCG
CGGACGCGGGCCTGGGGCGTGAAAGCCTGTACAAGGCCATCAGCCCGGGCGCACATCCACGCTACGAAACCGTCCAGAAA
ATCGTGCGCGCATTGGGCGTCAGGTTGAACGTGACGCCCGCTTGA

Structures

Toxin

• AlphaFold DB: F6G268

Antitoxin

• AlphaFold DB: F6G269