Detailed information of TA system TA057466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1339939..1340572
• Replicon: chromosome
• Accession: NZ_CP012939
• Organism: Ralstonia solanacearum strain UW163

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F6FXL1
• Locus tag: UW163_RS06210
• Protein ID: WP_014615899.1
• Coordinates: 1340159..1340572 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F6FXL0
• Locus tag: UW163_RS06205
• Protein ID: WP_003263271.1
• Coordinates: 1339939..1340172 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UW163_RS06190	1335513..1336802	-	1290	WP_003263268.1	MFS transporter	-
UW163_RS06195	1336810..1338078	-	1269	WP_124994693.1	AGE family epimerase/isomerase	-
UW163_RS06200	1338274..1339881	+	1608	WP_039567295.1	glucan biosynthesis protein	-
UW163_RS06205	1339939..1340172	+	234	WP_003263271.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
UW163_RS06210	1340159..1340572	+	414	WP_014615899.1	PIN domain-containing protein	Toxin
UW163_RS06215	1340547..1341173	-	627	WP_014615900.1	DUF1415 family protein	-
UW163_RS06220	1341384..1341758	+	375	WP_014615901.1	hypothetical protein	-
UW163_RS06225	1341833..1343329	-	1497	WP_014615902.1	glycerol kinase GlpK	-
UW163_RS06230	1343501..1345237	-	1737	WP_003266149.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-40)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14919.23 Da
• Isoelectric Point: 5.9701

>T57466 WP_014615899.1 NZ_CP012939:1340159-1340572 [Ralstonia solanacearum]
MPAAEAFFDSNIVLYLLSADAAKADAAEALLMAGGVVSVQVLNETTHVMRRKLAMPWHAIEAVQEAVRAQCRVEPLTVET
HDLGRHLAQRYGLSVYDALIVAAASLAGCRVLYSEDMQHGLVIEQRLRVVNPFFASA

Nucleotide

• Length: 414 bp

>T57466 NZ_CP012939:1340159-1340572 [Ralstonia solanacearum]
ATGCCCGCGGCTGAGGCGTTTTTCGACAGCAACATCGTGCTGTACCTGCTGTCGGCCGATGCCGCCAAGGCCGATGCGGC
GGAAGCGCTACTGATGGCCGGCGGCGTCGTCAGCGTGCAGGTGCTCAACGAAACGACGCACGTGATGCGCCGCAAGCTCG
CCATGCCGTGGCACGCGATCGAAGCGGTGCAGGAAGCCGTACGCGCGCAATGCCGGGTCGAGCCGCTGACGGTCGAAACC
CATGATCTCGGGCGGCATCTTGCGCAGCGCTATGGATTGTCGGTCTATGACGCGCTGATCGTCGCCGCAGCGTCGCTGGC
CGGTTGCCGCGTCCTGTATTCCGAGGACATGCAGCACGGCCTGGTGATCGAGCAGCGTCTGCGCGTCGTCAATCCTTTTT
TCGCGTCGGCGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8664.82 Da
• Isoelectric Point: 8.7288

>AT57466 WP_003263271.1 NZ_CP012939:1339939-1340172 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALGLHEGDDVEVVVAGERSFGVRRKRSARELFERLHQYRGKLPADFIFERDDANARG

Nucleotide

• Length: 234 bp

>AT57466 NZ_CP012939:1339939-1340172 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCCATCCGGCTGCCCGCAGCCGTTGTGGAGGCGCTTGGACTGCACGAAGG
CGACGACGTCGAGGTGGTGGTGGCGGGCGAACGCTCGTTTGGTGTGCGCCGCAAGCGTTCCGCGCGTGAGTTGTTTGAGC
GGCTCCACCAATACCGCGGCAAGCTGCCCGCCGACTTCATCTTCGAGCGCGACGACGCCAATGCCCGCGGCTGA

Structures

Toxin

• AlphaFold DB: F6FXL1

Antitoxin

• AlphaFold DB: F6FXL0