Detailed information of TA system TA057447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 5783702..5785123
• Replicon: chromosome
• Accession: NZ_CP012901
• Organism: Pseudomonas aeruginosa strain N15-01092

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: AOY09_RS27655
• Protein ID: WP_025297643.1
• Coordinates: 5783702..5784775 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: AOY09_RS27660
• Protein ID: WP_158025315.1
• Coordinates: 5784830..5785123 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY09_RS27630	5779795..5780223	-	429	WP_025297641.1	S24 family peptidase	-
AOY09_RS27635	5780503..5782086	+	1584	WP_025297642.1	hypothetical protein	-
AOY09_RS27640	5782139..5782435	-	297	WP_023128612.1	hypothetical protein	-
AOY09_RS27650	5782733..5782987	-	255	WP_023128610.1	hypothetical protein	-
AOY09_RS27655	5783702..5784775	+	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
AOY09_RS27660	5784830..5785123	+	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
AOY09_RS27665	5785336..5786031	-	696	WP_196747054.1	transporter substrate-binding domain-containing protein	-
AOY09_RS27670	5786030..5786224	+	195	WP_023128607.1	OprD family outer membrane porin	-
AOY09_RS27675	5786448..5786812	-	365	Protein_5339	DNA binding protein	-
AOY09_RS27680	5787143..5787721	-	579	WP_025297644.1	hypothetical protein	-
AOY09_RS27685	5787803..5788186	-	384	WP_025297645.1	hypothetical protein	-
AOY09_RS27690	5788189..5788659	-	471	WP_023128604.1	hypothetical protein	-
AOY09_RS27695	5788702..5789097	-	396	WP_023128603.1	hypothetical protein	-
AOY09_RS27700	5789262..5789717	+	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
AOY09_RS27705	5789808..5790020	-	213	WP_023128602.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5777147..5798951	21804

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T57447 WP_025297643.1 NZ_CP012901:5783702-5784775 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T57447 NZ_CP012901:5783702-5784775 [Pseudomonas aeruginosa]
GTGACTGAACGCGACTACAAAAACATCAACCTCAAACGATGCATTATCGAGGAAACGACTCATTGCTTCATCCGAGATAA
TGGCCTGAAGCTGCGATCCTATGGTGACAAGCCTGGGAGTTCCGGTCTTCGAGTAGTCTTCGGAAAAGCAGGGATTGAAG
ATGCCACGCTTGATATTTTTTTCACGAATGTCGGTGCGTCTACCCTGACCTACAAGGTGGGCAAGAACCAAGCTCTCGGC
CAGCAGCTAGCGGATGCTCTTTACGAAACAATCCACCCCGATGAATTCGTAACGATGAACCTTGGTATCAAAGGCGTCGA
TCTGGATGATGCCGAAGCCCTTATCAAGGAACTCACTGAGTCAGCCAATGCGGACATTGAGGTTGCTTCGCGCACAAGCG
CTGATCGCAAGCATATTTGGAAGCTCAAGTCTAAAAAGAACTTGGATGAGTTGACTGTCACTCAGCACGAGAACACCCAT
AAACTGCAGATCCAGGGTCGTCCTCTCAGCTGCTATCAGCAGTTGAGCTACCTGCTCACCGAGCTCCTAGAGATCAACGC
ACTCGAATGCATCCTGTTCAAAAAGGAAGAAAATCGCTCGGAGTTCGTTTGTGCCGAGGTCGCGGAAAGCGTACTCAAGG
AAAGGTTTGGCGACTGCTACGCCAACCTGCCACAGACCTCGCGTAGGCTGCTCCTGGCAAGCCTTTGCGTGAGGCTTGCC
AGCCCCGCGCTACCCGACTACTGCATGCTGGTTTATCCGGAACTCCGCACCCTTGAAGGCGCGATCAAACAGAAGCTTGC
AGAAAAAAACCTTTCAAATCAGGAAGAAAGCTTCGGTGGCTTCTTTACCAAGACCAATGGTCAGTTTTATCTGAAGCAGC
AATACCATGCTGATGTCGGCGATGCTAAACTACAGGGTCATCTGGACAACGCTTACACCTTCTTCAACAAGCAACGCCAC
GGATTGTTCCACATGGAAGAGATGCCATCCGTTAGTCGAATGATTAGCAACATCACCCAAGCTGTGTCATTGTGTGATGA
AGCATATGATCATATCAAGAATTTGTATAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT57447 WP_158025315.1 NZ_CP012901:5784830-5785123 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT57447 NZ_CP012901:5784830-5785123 [Pseudomonas aeruginosa]
ATGACGGTAGTTACCGCTACCAGTTATGAGAACCCCTTATCTCGTCTTTCTATTATTGCTAGCGAGATGCGCAATACATC
TCACTCCAGCAAGGAGATAGTTCTATTCGATCTACTCTGCTCTAACGGAGAAGAGTGGAATCGTTTCGTCTCCATCAATT
ACAACGGCACCGACTTCGAAAAAAGCACCTGTAGCATTGTCTCCAAGTCCGACATCCCTACAGACCTCTTGGAAACACAA
ACTCGCTTCTTTCAAATCCATCCACAATATCTGCTTGATTCAGTTTTGAATTAA